T4019
Trypsin−Agarose
buffered aqueous suspension, from bovine pancreas trypsin
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About This Item
Empfohlene Produkte
Biologische Quelle
bovine pancreas (trypsin)
Form
buffered aqueous suspension
Konzentration
≥15 units/mL (packed gel)
Kennzeichnungsgrad
≥15 units per mL (packed gel)
Matrix
beaded agarose
Lagertemp.
2-8°C
Verwandte Kategorien
Allgemeine Beschreibung
The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.
Anwendung
An experimental approach using SDS-PAGE, in-gel trypsin digestion and proteomic analysis identified five endosomal proteins. Trypsin cleavage of the high-risk HPV-16 E5 protein (16E5) generates a unique four-amino acid C-terminal peptide that serves as a marker for E5 expression in transfected cells and epithelial cell lines containing integrated and episomal HPV-16 DNA.
Einheitendefinition
One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 °C (titrimetric assay).
Physikalische Form
Suspension in approx. 10 mM acetic acid, pH 3.2, containing preservative
Angaben zur Herstellung
TPCK-treated
Signalwort
Warning
H-Sätze
Gefahreneinstufungen
Skin Sens. 1
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
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