P1253

Phosvitin aus Eigelb

• CAS-Nummer: 9008-96-2
• UNSPSC Code: 12352202
• NACRES: NA.81
• MDL number: MFCD00131908
• Form: powder
• Biological source: egg yolk

Eigenschaften

• biological source: egg yolk
• Quality Level: 200
• form: powder
• storage temp.: −20°C

Beschreibung

General description

Phosvitin from egg yolk is a polyanionic phosphoserine rich protein.[1] This phosphoglycoprotein comprises α-helices and β-sheets structural elements, which are poorly organized due to the presence of phosphoserine.[1] It represents about 11% of yolk proteins and is hydrophilic.[2]

Application

Phosvitin from egg yolk has been used:

• as a control protein for immobilization on-chip for deep purple protein staining methods for monitoring post-translation modifications[3]
• as a substrate for casein kinase I_ε in in vitro casein kinase assay[4]
• as a standard in gel filtration chromatography (GFC) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for quantification of phosvitin from egg extracts[5]
• for immobilization onto Layer-by-Layer (LbL) for Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR) studies[6]

Biochem/physiol Actions

Phosvitin fed to rats by adding egg yolk to their food resulted in decreased absorption of magnesium, iron and calcium but not of phosphorus. [7]

Phosvitin from egg yolk is a calcium chelator[1] and binds to iron present in the yolk.[2] The metal-binding functionality is exploited in mineral-binding bioactive peptide production.[2] It is a nutraceutical and has potential for phosphopeptides production.[2]

Other Notes

A phosophoprotein containing 8-10% phosphorus. Molar N/P ratio approx. 2.7.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)