L0777

Lipase aus Aspergillus oryzae

solution, ≥100,000 U/g, white, beige

• Synonym(e): AOL, Lipolase 100L
• CAS-Nummer: 9001-62-1
• EC-Nummer: 3.1.1.3.
• EG-Nummer: 232-619-9
• MDL-Nummer: MFCD00131509
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: Aspergillus sp. (Aspergillus oryzae)
• Form: solution
• Spezifische Aktivität: ≥100,000 U/g
• Lagerbedingungen: (Tightly closed. Dry)
• Methode(n): cell based assay: suitable
• Farbe: beige; white
• UniProt-Hinterlegungsnummer: I8ABR3; I8ADK3
• Lagertemp.: 2-8°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• InChIKey: QWZUIMCIEOCSJF-CHHCPSLASA-N

Beschreibung

Allgemeine Beschreibung

Research area: Cell Signaling

Lipase is derived from Aspergillus oryzae by ammonium sulfate precipitation and chromatography.Lipases or triacylglycerol acyl hydrolases are a group of hydrolase enzymes that are usually found in humans and animals with monogastric stomachs. Lipase enzymes are generally formed in the pancreas and stomach where they aid in fat and lipids digestion.(2)

Anwendung

Lipase from Aspergillus oryzae has been used:

• as a control enzyme in activity assays
• as a component in lipolase stock solution for the hydrolysis experiments
• to incubate scaffolds for lipase-accelerated degradation experiments
• as the lipolytic enzyme standard to detect lipolytic enzymatic activity via chromogenic agar plates and zymography

Biochem./physiol. Wirkung

Lipase is widely used in several industries including food and pharmaceuticals. It mediates the hydrolysis of fats and oil. Lipase is utilized for peptide synthesis and in the detergent industry. It is active in the range of pH 2-5 and temperature between 30-50°C. Metal ions such as Fe²⁺, Fe^3+, and Cu²⁺ prevent the action of lipase. A less polar organic solvent is preferred for high stability.Lipases play a crucial role in digestion as well as the transportation and processing of dietary lipid substrates by catalyzing the hydrolysis of ester bonds in lipid substrates. The lipase from Aspergillus oryzae (AOL), a multipurpose biocatalyst can be used in the kinetic resolution of a biotin intermediate lactone,α-lipoic acid, and 1-phenylethanol. Additionally, it has the ability to stereoselectively catalyze the hydrolysis of ethyl 2-(4-hydroxyphenoxy) propanoate and its analogs, which are key intermediates in the production of aryloxyphenoxypropionate herbicides. AOL serves as a promising biocatalyst in the esterification of a series of short-chain acids and alcohols to produce flavor esters. It can be used to catalyze the esterification of lauric acid with aromatic alcohol-benzyl alcohol.

Angaben zur Herstellung

Produced by submerged fermentation of a genetically modified Aspergillus oryzae microorganism

Rechtliche Hinweise

Ein Produkt der Novozyme Corp.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H334
• P-Sätze: P261 - P284 - P501
• Gefahreneinstufungen: Resp. Sens. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)