β-Estradiol 6-((O-carboxy-methyl)oxime: BSA, Product Number E5630, is soluble in PBS to 0.90-1.10 mg/mL, and this will yield a faintly hazy to hazy, colorless to faintly yellow solution. Accordingly, E5630-5MG is soluble in 4.5-5.6 mL of PBS. The actual batch-specific solubility is listed on the corresponding CoA. Please download the CoA from the “Documents” section of the PDP at the following link:
https://www.sigmaaldrich.com/product/sigma/e5630#product-documentation
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E5630
β-Estradiol 6-(O-carboxymethyl)oxime: BSA
mitogen-activated protein kinase (MAPK) stimulator, lyophilized powder
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 5 mg | Warenkorb auf Verfügbarkeit prüfen | CHF 213.00 | |
| 10 mg | Warenkorb auf Verfügbarkeit prüfen | CHF 349.00 |
Über diesen Artikel
Form:
lyophilized powder
Quality level:
Technischer Dienst
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Unterstützung erhaltenProduktname
β-Estradiol 6-(O-carboxymethyl)oxime: BSA,
sterility
non-sterile
Quality Level
form
lyophilized powder
extent of labeling
~30 mol steroid per mol BSA
solubility
phosphate buffer: 0.90-1.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow
shipped in
ambient
storage temp.
2-8°C
General description
Application
β-Estradiol 6-(O-carboxymethyl)oxime: BSA has been used:
- to test its effect on the N-methyl-D-aspartate receptor (NMDAR) currents in female dorsal root ganglion (DRG) neurons[2]
- to coat microplates for the detection of plasma 17β-Estradiol levels using indirect enzyme-linked immunosorbent assay (ELISA)[3]
- to test its effect on MutL homolog 1 in colorectal cancer lines[4]
Biochem/physiol Actions
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| form lyophilized powder | form lyophilized powder | form powder | form powder |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| solubility phosphate buffer: 0.90-1.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow | solubility 0.1 M phosphate buffer: 0.98-1.02 mg/mL, clear, yellow to orange (pH: 7.5) | solubility ethanol: 19.60-20.40 mg/mL, clear, colorless to faintly yellow | solubility 0.1 M NaOH: 10 mg/mL, clear to slightly hazy, colorless to faintly yellow |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| extent of labeling ~30 mol steroid per mol BSA | extent of labeling 3-5 mol FITC per mol BSA, 5-10 mol steroid per mol BSA | extent of labeling - | extent of labeling - |
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 2
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type P3 (EN 143) respirator cartridges
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Adrián Tintos et al.
Ecotoxicology and environmental safety, 66(2), 139-147 (2006-02-10)
To assess the effects of naphthalene on liver intermediary metabolism and plasma steroid hormones, immature female rainbow trout (Oncorhynchus mykiss), in a first experiment, were intraperitoneally injected (2 microLg(-1)) with vegetable oil alone (control) or containing naphthalene (10 and 50
Sun Zhang et al.
Acta pharmacologica Sinica, 26(2), 171-176 (2005-01-25)
To investigate the mechanism of the action of estrogen, which stimulates the release of secreted amyloid precursor protein alpha (sAPP(alpha)) and decreases the generation of amyloid-beta protein (A(beta)), a dominant component in senile plaques in the brains of Alzheimer's disease
Sex-dependent differences in the activity and modulation of N-methyl-d-aspartic acid receptors in rat dorsal root ganglia neurons.
McRoberts, et al.
Neuroscience, 148, 1015-1020 (2018)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| E5630-5MG | 04061832849966 |
| E5630-10MG | 04061833604359 |
| E5630-25MG | 04061832849959 |
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Buongiorno, il datasheet incluso con il prodotto E56-30 non è chiaro. Potrei sapere il volume finale in cui sciogliere 5mg di E2/BSA e la relativa concentrazione?
1 answer-
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What is the solution stability of Product E5630, β-Estradiol 6-(O-carboxymethyl)oxime: BSA?
1 answer-
We have not performed solution stability studies, and have found no information on solution stability in the literature. We would therefore recommend using freshly prepared solutions.
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How is Product E5630, β-Estradiol 6-(O-carboxymethyl)oxime: BSA, prepared?
1 answer-
Our method of preparation is considered proprietary information However, a method of preparation (not necessarily Sigma-Aldrich’s) has been described in J. Biol. Chem., 228, 713-727 (1957).
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What is the solubility of Product E5630, β-Estradiol 6-(O-carboxymethyl)oxime: BSA?
1 answer-
We have solubilized the product in phosphate buffered saline at 1 mg/mL. Solutions may be somewhat hazy.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the shelf life of Product E5630, β-Estradiol 6-(O-carboxymethyl)oxime: BSA?
1 answer-
There is no assigned shelf life date for the product. The guarantee of quality is one year from the time customer receives the product for unopened bottles stored at 2-8°C.
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What is the molecular weight of Product E5630, β-Estradiol 6-(O-carboxymethyl)oxime: BSA?
1 answer-
The Certificate of Analysis has lot specific information for the number of moles of steroid per mole of bovine serum albumin. From this data and the molecular weights of the components, an approximate molecular weight for the compound can be calculated. For example, if the moles of beta estradiol to BSA is 32, then the approximate molecular weight for the compound is 76.4 kDa (BSA has a molecular weight of approximately 66.4 kDa and 32 moles of estradiol is approximately 10 kDa more).
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