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D2886

Sigma-Aldrich

DNA-Ligase aus T4-infizierten E. coli

buffered aqueous glycerol solution

Synonym(e):

Polydeoxyribonucleotid-Synthase, Polynucleotid-Ligase, T4 DNA-Ligase

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About This Item

CAS-Nummer:
9015-85-4
EC-Nummer:
6.5.1.1 (BRENDA, IUBMB)
EG-Nummer:
232-770-0
MDL-Nummer:
MFCD00163508
UNSPSC-Code:
12352204
NACRES:
NA.53

Qualität

for molecular biology

Qualitätsniveau

200

Form

buffered aqueous glycerol solution

Spezifische Aktivität

4,000 U/mL

Mol-Gew.

68 kDa

UniProt-Hinterlegungsnummer

P00970

Lagertemp.

−20°C

Angaben zum Gen

bacteriophage T4 ... 30(1258680)

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Verwandte Kategorien

Anwendung

Suitable for:
  • Ligation of blunt ended or cohesive DNA fragments
  • Ligation of cloning vector and restriction insert fragments
  • Seal nicks in double stranded DNA and RNA or DNA/RNA hybrids
  • Couple RNA single strands by bridging oligonucleotide adapters

Biochem./physiol. Wirkung

T4 DNA Ligase forms an energy dependent phosphodiester linkage between the termini of adjacent polynucleotides of duplex DNA. The ligation reaction requires ATP as a cofactor. Ligation of blunt-ended fragments requires higher enzyme concentration and can be facilitated by using PEG in the reaction mixture. The enzyme requires a 3′ hydroxyl and 5′ phosphate for ligation. Self-ligation of vector DNA can be prevented by dephosphorylation with alkaline phosphatase. T4 ligase plays an active role in repair of DNA and RNA nicks.

Komponenten

T4 DNA Ligase is supplied in a solution containing 20 mM Tris-HCl (pH 7.5), 50 mM KCl, 1 mM DTT, and 50% (v/v) glycerol.

Einheitendefinition

One Weiss unit is defined as the amount of enzyme required to catalyze the exchange of 1 nmole of P32 from pyrophosphate into ATP as Norit-absorbable material in 20 minutes at 37°C.

Sonstige Hinweise

T4 DNA Ligase is inactivated by heating at 65 °C for 10 minutes.

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Piktogramme

Health hazard
GHS08

Signalwort

Danger

H-Sätze

H334

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

Adam B Robertson et al.
The Journal of biological chemistry, 287(39), 32953-32966 (2012-08-01)
The Escherichia coli very short patch (VSP) repair pathway corrects thymidine-guanine mismatches that result from spontaneous hydrolytic deamination damage of 5-methyl cytosine. The VSP repair pathway requires the Vsr endonuclease, DNA polymerase I, a DNA ligase, MutS, and MutL to
Justin L Sparks et al.
Molecular cell, 47(6), 980-986 (2012-08-07)
Ribonucleotides are incorporated into DNA by the replicative DNA polymerases at frequencies of about 2 per kb, which makes them by far the most abundant form of potential DNA damage in the cell. Their removal is essential for restoring a
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 1-1 (1989)
Athena Kantartzis et al.
Cell reports, 2(2), 216-222 (2012-09-04)
Trinucleotide repeat (TNR) expansions are the underlying cause of more than 40 neurodegenerative and neuromuscular diseases, including myotonic dystrophy and Huntington's disease. Although genetic evidence points to errors in DNA replication and/or repair as the cause of these diseases, clear
P Upcroft et al.
Gene, 51(1), 69-75 (1987-01-01)
We describe a system to generate cDNA or genomic libraries from DNA segments that have blunt termini. Background and rearrangement levels are low, but efficiencies are high and the procedural times very short. T4 ligase in the presence of polyethylene
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