C7604

Anti-γ-Tubulin-Cy3^™ antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-γ-Tubulin
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: CY3 conjugate
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen 48 kDa
• Speziesreaktivität: human, chicken
• Methode(n): direct immunofluorescence: 1:200 using methanol/acetone-fixed chicken fibroblats cells or HeLa cells (human).
• UniProt-Hinterlegungsnummer: P23258
• Versandbedingung: wet ice
• Lagertemp.: 2-8°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... TUBG1(7283) , TUBG2(27175)

Beschreibung

Allgemeine Beschreibung

γ-Tubulin (48 kDa) is a widely expressed and highly conserved protein within the microtubule organizing centers (MTOCs) or centrosome in eukaryotic cells.

Tubulin, γ 1 is a protein encoded by the TUBG1 gene in humans. This gene encodes a member of the tubulin superfamily. γ-tubulin interacts with Arp2/3, a complex that nucleates actin filaments and with the Arp2/3 activator WASH. It is found to have relationship with actin filament dynamics.

Immunogen

synthetic peptide corresponding to the N-terminal region of human γ-tubulin (amino acids 38-53, with C-terminally added lysine) conjugated to KLH. This sequence is specific for γ-tubulin and not found in other members of the tubulin family such as α− β−, δ and ε− tubulins. This sequence is identical in mouse and rat γ-tubulin and highly conserved among species (Drosophila, Aspergillus, and yeast γ-tubulin).

Anwendung

Anti-γ- Tubulin-Cy3 antibody produced in rabbit has been used in direct immunofluorescence (IF)and immunofluorescent staining.

Anti-γ-Tubulin-Cy3^™ antibody produced in rabbit is suitable for direct immunofluorescence at a dilution of 1:200 using methanol/acetone-fixed chicken fibroblats cells or HeLa cells (human).

Biochem./physiol. Wirkung

γ-Tubulin binds the microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome.

γ-Tubulins in the γ-TuRC might promote microtubule nucleation by providing a template that enhances the intrinsically weak lateral interaction between αβ-tubulin heterodimers when associated laterally. It has a unique functional property of a monomeric tubulin. γ-tubulin is also found to be expressed in lung cancer. Cep70 as an important centrosomal protein that interacts with γ-tubulin and plays a critical role in the regulation of mitotic spindle assembly.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Rechtliche Hinweise

Cy is distributed under license from Amersham Biosciences Limited.

Cy3 is a trademark of Cytiva

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable