C4018

Choloylglycine Hydrolase from Clostridium perfringens (C. welchii)

lyophilized powder, ≥100 units/mg protein

• Synonym(e): 3α,7α,12α-Trihydroxy-5β-cholan-24-oylglycine amidohydrolase
• CAS-Nummer: 37289-07-9
• UNSPSC Code: 12352204
• NACRES: NA.54
• EG-Nummer: 3.5.1.24 (BRENDA, IUBMB)
• MDL number: MFCD00130789
• Specific activity: ≥100 units/mg protein

Eigenschaften

• form: lyophilized powder
• Quality Segment: 200
• specific activity: ≥100 units/mg protein
• composition: Protein, ≥50% biuret
• shipped in: wet ice
• storage temp.: −20°C

Beschreibung

Application

The enzyme from Sigma has been used in the analysis of bile samples in various studies.[1][2]

Biochem/physiol Actions

The enzyme is an N-terminal nucleophilic (Ntn) hydrolase that catalyzes the hydrolysis of amide bonds, liberates the glycine/taurine moiety from the steroid core and eventually yields unconjugated bile acids. Agents that oxidize thiol groups (e.g., p-mercuribenzoate, iodoacetamide, Hg²⁺, Cu²⁺, and Cd²⁺) have been shown to strongly inhibit bile salt hydrolase (BSH) activity in Clostridium perfringens.[3]

Physical form

Partially purified lyophilized powder containing buffer salts and stabilizer

Other Notes

One unit will hydrolyze 1.0 μmole of glycocholic acid to glycine and cholic acid in 5 min at pH 5.6 at 37 °C.

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)