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B6149
5-Brom-4-Chlor-3-Indolylphosphat Dinatriumsalz
chromogenic, ≥98% (HPLC), powder
Synonym(e):
BCIP®, X-phosphat Dinatriumsalz
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 25 mg | Warenkorb auf Verfügbarkeit prüfen | CHF 49.90 CHF 42.41 | |
| 50 mg | Warenkorb auf Verfügbarkeit prüfen | CHF 92.00 | |
| 100 mg | Warenkorb auf Verfügbarkeit prüfen | CHF 108.00 | |
| 500 mg | Warenkorb auf Verfügbarkeit prüfen | CHF 490.00 | |
| 1 g | Warenkorb auf Verfügbarkeit prüfen | CHF 537.00 CHF 456.45 | |
| 5 g | Warenkorb auf Verfügbarkeit prüfen | CHF 2’960.00 |
Über diesen Artikel
Lineare Formel:
C8H4BrClNO4P · 2Na
CAS-Nummer:
Molekulargewicht:
370.43
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83
MDL number:
CHF 42.41
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Unterstützung erhaltenProduktname
5-Brom-4-Chlor-3-Indolylphosphat Dinatriumsalz, ≥98% (HPLC)
Quality Level
assay
≥98% (HPLC)
form
powder
solubility
H2O: 20 mg/mL, DMF: insoluble
storage temp.
−20°C
SMILES string
[Na+].[Na+].[O-]P([O-])(=O)Oc1c[nH]c2ccc(Br)c(Cl)c12
InChI
1S/C8H6BrClNO4P.2Na/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;;/h1-3,11H,(H2,12,13,14);;/q;2*+1/p-2
InChI key
OAZUOCJOEUNDEK-UHFFFAOYSA-L
General description
Histochemisches Substrat für alkaline Phosphatase.
Application
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
For the colorimetric detection of alkaline phosphatase-labeled molecules.
5-Bromo-4-chloro-3-indolyl phosphate disodium salt has been used to detect protein-antibody complexes. It has also been used for tissue staining in alkaline phosphatase histochemistry.
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
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Dieser Artikel | |||
|---|---|---|---|
| assay ≥98% (HPLC) | assay ≥99% (HPLC) | assay ≥98.0% (T) | assay ≥99.5% (T) |
| solubility H2O: 20 mg/mL, DMF: insoluble | solubility DMF: 20 mg/mL, clear to slightly hazy, yellow | solubility water: soluble 50 mg/mL at 25 °C | solubility H2O: 0.5 M at 20 °C, clear, colorless |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 200 |
| form powder | form powder | form powder | form coarse powder, fine crystals |
| storage temp. −20°C | storage temp. −20°C | storage temp. - | storage temp. - |
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Artikel
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
Verwandter Inhalt
Manli Qi et al.
Journal of bacteriology, 193(10), 2498-2509 (2011-03-29)
The Chlamydia-specific hypothetical protein CT795 was dominantly recognized by human antisera produced during C. trachomatis infection but not by animal antisera raised against dead chlamydia organisms. The immundominant region recognized by the human antibodies was mapped to the N-terminal fragment
P E Høyer et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 44(8), 855-863 (1996-08-01)
We describe a quantitative histochemical method for demonstration of five N-acetyl-glucosamine binding lectins in the syncytiotrophoblast of human term placenta. The method employs biotinylated lectins and alkaline phosphatase-conjugated avidin. The alkaline phosphatase activity is detected by using 5-bromo-4-chloro-indoxyl phosphate as
Thermal preference ranges correlate with stable signals of universal stress markers in Lake Baikal endemic and Holarctic amphipods
Axenov-Gribanov D, et al
PLoS ONE, 11(10), e0164226-e0164226 (2016)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| B6149-25MG | 04061833434482 |
| B6149-100MG | 04061833434468 |
| B6149-5G | 04061833434499 |
| B6149-50MG | 04061835517688 |
| B6149-1G | 04061833434475 |
| B6149-500MG | 04061835553921 |