This product has not been tested specifically for use in cell staining on slides or coverslips. However, it has been used successfully in staining osteoblasts by other customers. Cell are fixed prior to staining. Suitability for use with a specific cell type or for fresh smears would have to be determined by the end user. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/111/390/b1911dat-ms.pdf
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 100 mL | Ab HEUTE lieferbarvonSchnelldorf | CHF 109.00 |
Über diesen Artikel
CHF 109.00
Ab HEUTE lieferbarDetails
Produktname
BCIP®/NBT Flüssigsubstrat-System, ready to use solution
Quality Segment
form
liquid
storage temp.
2-8°C
Application
- zur Färbung alkalischer Phosphatase (ALP) in Knochenmarkstromazellen
- als Substrat beim Western-Blot-Detektion von MMP2-(Matrix-Metalloproteinase-2-)spezifischen Antikörpern
- als Substrat für alkalische Streptavidin-Phosphatase in ELISpot-Assays (Enzyme-Linked-Immuno-Spot-Assays)
Legal Information
Disclaimer
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|---|---|---|---|
| Quality Level 300 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| form liquid | form liquid | form liquid | form liquid |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
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Lagerklasse
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protokolle
BCIP/NBT substrate produces stable, intense color for alkaline phosphatase assays, ideal for visual observation.
Artikel
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
Verwandter Inhalt
Global Trade Item Number
| SKU | GTIN |
|---|---|
| B1911-100ML | 04061838251626 |
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can the product be used for citological procedures? specifically, air dry citological slides?
1 answer-
Helpful?
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Should this be applied to fixed cells or live cells for staining?
1 answer-
Product B1911 is not formulated as a balanced salt solution. Therefore, it should be applied to fixed cells, as live cells are not suitable for staining with this solution.
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Is the BCIP in your BCIP/NBT Liquid Substrate part number B1911 5-bromo-4-chloro-3'-indolyphosphate p-toluidine salt?
1 answer-
The BCIP used in Product B1911 is not 5-bromo-4-chloro-3'-indolyphosphate p-toluidine salt (CAS# 6578-06-9).
Helpful?
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