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B3679

Sigma-Aldrich

BCIP®/NBT-Blue

alkaline phosphatase substrate, chromogenic, liquid

Synonym(e):

5-bromo-4-chloro-3-indolyl-phosphate / Nitro blue tetrazolium chloride liquid substrate system

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About This Item

UNSPSC-Code:
12352204
NACRES:
NA.83

product name

BCIP®/NBT-Purple Liquid Substrate System for Membranes, ready to use alkaline phosphatase substrate

Qualitätsniveau

100

Form

liquid

Lagertemp.

2-8°C

Verwandte Kategorien

Anwendung

BCIP®/NBT-Purple Liquid Substrate System for Membranes has been used as a substrate for alkaline phosphatase
  • for the detection of secondary antibodies by immunoblot
  • in ELISPOT assay
  • in dot blot analysis
  • in western blot to visualize protein bands on nitrocellulose membrane

Biochem./physiol. Wirkung

5-bromo-4-chloro-3-indolyl phosphate (BCIP)/ nitro blue tetrazolium (NBT) is a chromogenic substrate system for the detection of alkaline phosphatase (AP) enzyme. AP hydrolyses BCIP to form an intermediate leucoindigo compound and is further oxidized by NBT to form 5,5′-dibromo-4,4′-dichloro indigo (BCI). NBT is reduced to an insoluble purple diformazan (DF) and forms NBT-DF. A mixture of NBT-DF and BCI results in the dark purple colored precipitate. AP by hydrolysing many BCIP molecules makes it a highly sensitive method.

Vorsicht

Stable at least one year at 2-8 °C.

Physikalische Form

Ready-to-use.

Rechtliche Hinweise

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Piktogramme

Exclamation mark
GHS07

Signalwort

Warning

H-Sätze

H317

Gefahreneinstufungen

Skin Sens. 1

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Artikel

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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