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A9226

Alkaline Puffer -Lösung

1.5 M, pH 10.3 (25 °C)

Synonym(e):

Basic pH buffer

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PackungsgrößeSKUVerfügbarkeitPreis
100 mL
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CHF 117.00

Über diesen Artikel

NACRES:
NA.25
UNSPSC Code:
12161700

CHF 117.00

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Unterstützung erhalten

form

liquid

Quality Level

200

concentration

1.5 M

pH

10.3 (25 °C)

storage temp.

2-8°C

General description

Alkaline buffer solution, 1.5 M, pH 10.3 at 25 °C is used in alkaline phosphatase reactions. It contains 2-amino-2-methylpropanol (AMP) and is used in conjunction with p-nitrophenyl phosphate (pNPP) enzyme substrate.

Application

Alkaline buffer solution has been used to measure the alkaline phosphatase (ALP) activity in the:
  • rat bone marrow stromal cells (BMSC)[1]
  • osteoblast-like cells (SaoS-2, ATCC HTB 85)[2]
  • mouse MC3T3-E1[3]

Other Notes

Contains 2-amino-2-methylpropanol

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Dieser Artikel
S2404A4955487664
Alkaline buffer solution 1.5 M, pH 10.3 (25 °C)

Sigma-Aldrich

A9226

Alkaline buffer solution

Sodium acetate buffer solution pH 7.0±0.05 (25 °C), BioXtra, Molecular Biology, 3 M, non-sterile; 0.2 μm filtered

Sigma-Aldrich

S2404

Sodium acetate buffer solution

Alkaline Phosphatase Stabilizing Buffer liquid

Sigma-Aldrich

A4955

Alkaline Phosphatase Stabilizing Buffer

p-Nitrophenyl Phosphate Substrate Buffer This p-Nitrophenyl Phosphate Substrate Buffer is validated for use in ELISA.

Sigma-Aldrich

487664

p-Nitrophenyl Phosphate Substrate Buffer

concentration

1.5 M

concentration

3 M

concentration

-

concentration

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

form

liquid

form

liquid

form

liquid

form

liquid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

pH

10.3 (25 °C)

pH

7.0±0.05 (25 °C)

pH

-

pH

-

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H315,H318

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2

Lagerklasse

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
0000554200
0000554200
0000448293
0000448293
0000428687

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Global Trade Item Number

SKUGTIN
A9226-100ML04061838254689

Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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