A8200

Aminopeptidase from Aeromonas proteolytica

lyophilized powder, 50-150 units/mg protein

• Synonym(e): AAP, Aminopeptidase from Vibrio proteolyticus, bacterial leucyl aminopeptidase
• CAS-Nummer: 37288-67-8
• EG-Nummer: 3.4.11.10 (BRENDA, IUBMB)
• UNSPSC Code: 12352204
• EC Number: 232-874-6
• NACRES: NA.54
• MDL number: MFCD00166325
• Specific activity: 50-150 units/mg protein

Eigenschaften

• grade: Proteomics Grade
• Quality Segment: 300
• form: lyophilized powder
• specific activity: 50-150 units/mg protein
• mol wt: 29.5 kDa
• composition: Protein, ~40% biuret
• solubility: H₂O: soluble 0.9-1.1 mg/mL, clear, colorless
• foreign activity: endopeptidase, essentially free
• storage temp.: −20°C

Beschreibung

General description

A zinc-containing enzyme.

Application

Aminopeptidases are a family of widely distributed proteases, which may be used to study many significant biological processes such as protein maturation, hormone production, and peptide digestion. The enzyme has been used to measure the kinetic rate constant for the binding of bestatin, a general protease inhibitor, to aminopeptidase.[1]

Biochem/physiol Actions

Aminopeptidase from Aeromonas proteolytica is a metalloenzyme, which contains 2 atoms of Zn²⁺ in a single polypeptide with an approximate molecular weight of 29.5 kDa as determined by sedimentation. This enzyme has a high degree of stability, being stable even at temperatures of 70 °C for several hours. Partial inactivation occurs in 8 M urea. Maximum stability and activity are between pH 8.0-8.5. Aminopeptidase from Aeromonas proteolytica can function as an esterase.[2]

Aminopeptidase from Aeromonas proteolytica is involved in protein maturation, hormone production and peptide digestion.

Catalyzes the release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.

Physical form

Lyophilized powder containing tricine buffer, pH 8.0, zinc chloride and stabilizer.

Preparation Note

Dissolves in water at 0.9-1.1 mg/mL concentration to form a clear, colorless solution.

Other Notes

One unit will hydrolyze 1.0 μmole of L-leucine p-nitroanilide to L-leucine and p-nitroaniline per min at pH 8.0 at 25 °C.

Sicherheitshinweise

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)