A5159

Aminopterin

Hybri-Max^™, 50 ×, γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma

• Synonym(e): (S)-2-{4-[(2,4-Diaminopteridin-6-yl)-methylamino]-benzamido}-pentandisäure, 4-Amino-pteroyl-L-glutaminsäure, 4-Aminofolsäure, erythro-2-Amino-1-phenyl-1-propanol -hydrochlorid
• Empirische Formel (Hill-System): C₁₉H₂₀N₈O₅
• CAS-Nummer: 54-62-6
• Molekulargewicht: 440.41
• PubChem Substance ID: 24890950
• UNSPSC Code: 12352205
• MDL number: MFCD00036692
• NACRES: NA.75
• EC Number: 200-209-9
• Beilstein/REAXYS Number: 69045

Eigenschaften

• biological source: synthetic
• Quality Segment: 200
• grade: Hybri-Max^™
• sterility: γ-irradiated
• product line: BioXtra
• form: lyophilized powder
• concentration: 50 ×
• technique(s): cell culture | hybridoma: suitable
• impurities: endotoxin, tested
• solubility: 10 mL/vial, clear, red (in TC Medium)
• ε (extinction coefficient): 24,500 at 282 nm in 0.1 M NaOH at 1 M, 25,700 at 261 nm in 0.1 M NaOH at 1 M, 8,100 at 373 nm in 0.1 M NaOH at 1 M
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: Nc1nc(N)c2nc(CNc3ccc(cc3)C(=O)N[C@@H](CCC(O)=O)C(O)=O)cnc2n1
• InChI: 1S/C19H20N8O5/c20-15-14-16(27-19(21)26-15)23-8-11(24-14)7-22-10-3-1-9(2-4-10)17(30)25-12(18(31)32)5-6-13(28)29/h1-4,8,12,22H,5-7H2,(H,25,30)(H,28,29)(H,31,32)(H4,20,21,23,26,27)/t12-/m0/s1
• InChI key: TVZGACDUOSZQKY-LBPRGKRZSA-N
• Gene Information: human ... FPGS(2356) ; mouse ... Fpgs(14287)

Beschreibung

Application

Aminopterin concentrate supplement for hybridoma cell culture applications.

Biochem/physiol Actions

Folsäure Antagonist

Folic acid antagonist. Aminopterin is actively transported into cells by the folate transporter. In the cell, it is converted to a high molecular weight polyglutamate metabolite by folylpolyglutamate synthase that, in turn, binds to dihydrofolate reductase and inhibits its activity. Aminopterin-polyglutamate is degraded intracellularly by γ-glutamyl hydrolase.

Preparation Note

Reconstitute contents of vial with 10 mL sterile cell culture medium. Stock solution is sufficient to prepare 500 mL medium. Final working concentration: 0.4 μM aminopterin.

Other Notes

Wirksamer aber auch toxischer als Methotrexat

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Sicherheitshinweise

• pictograms: GHS06,GHS08
• signalword: Danger
• hcodes: H300,H315,H319,H341,H360FD
• pcodes: P202 - P264 - P270 - P301 + P310 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 1 Oral - Eye Irrit. 2 - Muta. 2 - Repr. 1B - Skin Irrit. 2
• Lagerklasse: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges