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Merck

30326

Sigma-Aldrich

EMPIGEN® BB Detergens

~30% active substance

Synonym(e):

N,N-Dimethyl-N-dodecylglycin-betain, N-(Alkyl C10-C16)-N,N-dimethylglycin-betain

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About This Item

Lineare Formel:
CH3(CH2)8-14CH2N+(CH3)2CH2COO-
CAS-Nummer:
Beilstein:
3670807
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12161900
PubChem Substanz-ID:
NACRES:
NA.25

Beschreibung

zwitterionic

Qualitätsniveau

Form

liquid

Zusammensetzung

solid content, 35.0-45.0 % (w/v)

Konzentration

~30% active substance

CMC

1.6-2.1 mM (20-25°C)

Dichte

1.030-1.060 g/mL at 20 °C

UV-Absorption

λ: 417 nm Amax: ≤0.055
λ: 441 nm Amax: ≤0.037

SMILES String

CCCCCCCCCCCC[N+](C)(C)CC([O-])=O

InChI

1S/C16H33NO2/c1-4-5-6-7-8-9-10-11-12-13-14-17(2,3)15-16(18)19/h4-15H2,1-3H3

InChIKey

DVEKCXOJTLDBFE-UHFFFAOYSA-N

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Anwendung

Empigen BB is a zwitterionic detergent compatible with anionic, non-ionic, cationic and other amphoteric surfactants.

Empigen BB is a useful detergent that has been used in the solubilization of membrane proteins and biochemical analysis of keratins. It not only helps to solubilize bigger amounts of protein but it also maintains antibody reactivity. It has been also used in the purification of leucine-rich repeat kinase 2 (LRRK2) in Escherichia coli during research in Parkinson′s disease. The use of Empigen in the preparation of viral antigens is being increasing due to its effectivity in giving greater antibody responses.

Rechtliche Hinweise

EMPIGEN is a registered trademark of Huntsman Corporation

Piktogramme

Exclamation mark

Signalwort

Warning

Gefahreneinstufungen

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Zielorgane

Respiratory system

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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Adam M Rotunda et al.
Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.], 30(7), 1001-1008 (2004-06-24)
Phosphatidylcholine injections are becoming an increasingly popular technique to treat localized fat accumulation. This formula is composed primarily of phosphatidylcholine and sodium deoxycholate, a bile salt used to solubilize the natural phospholipid in water. The mechanism through which this injectable
R Jennings et al.
Journal of medical virology, 31(2), 98-108 (1990-06-01)
HSV-1 antigen preparations solubilised from Vero cells by using either the non-ionic detergent Nonidet P40 or the zwitterionic detergent Empigen BB, and purified on sucrose density gradients or over a sucrose cushion, were tested by ELISA with anti-HSV-1 glycoprotein monoclonal
James K Wahl
Hybridoma and hybridomics, 21(1), 37-44 (2002-05-07)
Desmosomes are the most prominent cell-cell junctions in most epithelial cells and serve to link the intermediate filament cytoskeletons of adjacent cells. Desmogleins and desmocollins are the transmembrane core of the desmosome and both are members of the cadherin family
Wenjin Guo et al.
Protein expression and purification, 90(1), 34-39 (2013-05-15)
Vaccinia virus (VV) has been used to express a variety of heterologous proteins, including HIV envelope (Env) glycoproteins. The Env protein is synthesized as a precursor molecule, gp160, which is cleaved into the surface antigen gp120 and the transmembrane protein
Islam Khan et al.
Microbes and infection, 7(5-6), 853-859 (2005-05-10)
Campylobacter jejuni is a major food-borne pathogen and a leading cause of diarrhoea. A cytotoxin is most likely involved in the pathogenesis of inflammatory diarrhoea due to C. jejuni. A 45-kDa outer membrane protein encoded by the porA gene was

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