AB5152
Anti-Calcium Channel Antibody, Voltage Gated α 1A
Chemicon®, from rabbit
Synonym(e):
P/Q-type
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Über diesen Artikel
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
rat, mouse
Application:
ICC, IP, WB
Citations:
2
biological source
rabbit
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CACNA1A(773)
Immunogen
Purified peptide CNA1 from 865-881 of alpha 1A subunit of rat brain voltage-gated calcium channel (Starr et al. 1991) (Accession P54282) containing N-terminal lysine and tyrosine, conjugated to KLH with gluteraldehyde (Westenbroek et al. 1995).
Application
Anti-Calcium Channel Antibody, Voltage Gated α 1A is an antibody against Calcium Channel for use in IC, IP & WB.
In preparing full-length protein samples for Western blot or immunoprecipitation, the procedures should be performed at 4°C, with the following protease inhibitor mixture: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each)
Immunoprecipitation: 3 μg/mL
Immunocytochemistry: 5 μg/mL (1:60). Recommend subsequent staining with a labeled
second antibody.
Western blot: 1.5 μg/mL (1:200) using ECL
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
Immunoprecipitation: 3 μg/mL
Immunocytochemistry: 5 μg/mL (1:60). Recommend subsequent staining with a labeled
second antibody.
Western blot: 1.5 μg/mL (1:200) using ECL
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Recognizes Cav2.1 (alpha 1A subunit, P/Q-type of voltage-gated calcium channel, Cacna1a). Does not cross react with any other calcium channel proteins tested so far.
Preparation Note
Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution add glycerol (ASC gade or better at a ratio of 1:1 and maintain at -20°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Analysis Note
Control
CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 10 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 10 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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hcodes
pcodes
Hazard Classifications
Aquatic Chronic 3
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
Analysenzertifikate (COA)
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