07-353
Anti-Acetyl-Histon-H3(Lys14)-Antikörper
serum, Upstate®
Synonym(e):
H3K14Ac, Histone H3 (acetyl K14), H3 histone family, member T, histone 3, H3, histone cluster 3, H3
Größe auswählen
| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 100 μL | Warenkorb auf Verfügbarkeit prüfen | CHF 547.00 |
Über diesen Artikel
CHF 547.00
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
Saccharomyces cerevisiae, yeast, human
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable (ChIP-seq), dot blot: suitable, multiplexing: suitable, western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
acetylation (Lys14)
Gene Information
human ... H3C1(8350)
General description
Immunogen
Application
Ein unabhängiges Labor zeigte, dass dieser Antikörper Chromatin bevorzugt aus Wildtyp-Hefe immunpräzipitiert und nicht aus Hefestämmen mit einer Lysin-Substitution an Alanin an Rest 14.
Beadlyte Histon-Peptid-Spezifitätsassay:
Eine 1:1000-1:5000-Verdünnung einer früheren Charge wurde mit Histon H3-Peptiden, die verschiedene Modifikationen enthalten und an Luminex-Mikrosphären konjugiert sind, inkubiert. Es wurde keine Kreuzreaktivität mit Peptiden, die Acetyl-Lysin 9 oder Acetyl-Lysin 27 enthalten, nachgewiesen.
Histone
Epigenetik und Zellkernfunktion
Biochem/physiol Actions
Physical form
Preparation Note
Für eine maximale Rückgewinnung des Produkts das Fläschchen vor dem Entfernen des Deckels zentrifugieren.
Analysis Note
C6-Zelllysat, NIH/3T3-Zelllysat, humanes Brustkarzinom und humanes Lungenkarzinom.
Western-Blot-Analyse:
Eine 1:1.000-1:5.000-Verdünnung dieser Charge wies Acetyl-Histon H3 (Lys14) in Säureextrakten von mit Natriumbutyrat behandelten HeLa-Zellen (Katalognr. 17-305) nach.
Other Notes
Legal Information
Disclaimer
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| clone polyclonal | clone polyclonal | clone polyclonal | clone polyclonal |
| species reactivity Saccharomyces cerevisiae, yeast, human | species reactivity mouse, human, rat | species reactivity human, Saccharomyces cerevisiae, yeast, vertebrates | species reactivity mouse, rat, human |
| antibody form serum | antibody form purified immunoglobulin | antibody form serum | antibody form purified antibody |
| biological source rabbit | biological source rabbit | biological source rabbit | biological source rabbit |
| Gene Information human ... H3C1(8350) | Gene Information human ... HIST1H3F(8968) | Gene Information human ... H3C1(8350) | Gene Information human ... H3C1(8350) |
| technique(s) ChIP: suitable (ChIP-seq), multiplexing: suitable, dot blot: suitable, western blot: suitable | technique(s) ChIP: suitable (ChIP-seq), dot blot: suitable, flow cytometry: suitable, western blot: suitable | technique(s) ChIP: suitable (ChIP-seq), multiplexing: suitable, western blot: suitable | technique(s) ChIP: suitable (ChIP-seq), immunocytochemistry: suitable, western blot: suitable |
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Lagerklasse
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Verwandter Inhalt
Histone Modifications Poster
Cancer is a complex disease manifestation. At its core, it remains a disease of abnormal cellular proliferation and inappropriate gene expression. In the early days, carcinogenesis was viewed simply as resulting from a collection of genetic mutations that altered the gene expression of key oncogenic genes or tumor suppressor genes leading to uncontrolled growth and disease (Virani, S et al 2012). Today, however, research is showing that carcinogenesis results from the successive accumulation of heritable genetic and epigenetic changes. Moreover, the success in how we predict, treat and overcome cancer will likely involve not only understanding the consequences of direct genetic changes that can cause cancer, but also how the epigenetic and environmental changes cause cancer (Johnson C et al 2015; Waldmann T et al 2013). Epigenetics is the study of heritable gene expression as it relates to changes in DNA structure that are not tied to changes in DNA sequence but, instead, are tied to how the nucleic acid material is read or processed via the myriad of protein-protein, protein-nucleic acid, and nucleic acid-nucleic acid interactions that ultimately manifest themselves into a specific expression phenotype (Ngai SC et al 2012, Johnson C et al 2015). This review will discuss some of the principal aspects of epigenetic research and how they relate to our current understanding of carcinogenesis. Because epigenetics affects phenotype and changes in epigenetics are thought to be key to environmental adaptability and thus may in fact be reversed or manipulated, understanding the integration of experimental and epidemiologic science surrounding cancer and its many manifestations should lead to more effective cancer prognostics as well as treatments (Virani S et al 2012).
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 07-353 | 04053252588303 |