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U0508

Monoclonal Anti-Ubiquitin antibody produced in mouse

clone 6C1, ascites fluid

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Über diesen Artikel

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
Conjugate:
unconjugated
Clone:
6C1, monoclonal
Application:
ARR, ELISA (i), WB
Citations:
37
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
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biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

6C1, monoclonal

contains

15 mM sodium azide

species reactivity

human, rat, mouse, bovine

technique(s)

indirect ELISA: suitable, microarray: suitable, western blot: 1:1,000 using whole cell lysate of human promyelocitic HL60 cells

isotype

IgG2a

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

General description

Ubiquitin, an 8.5kD (approx.) 76 amino acid containing protein, is highly conserved and found in both prokaryotes and eukaryotes. It facilitates protein degradation in ubiquitin/proteasome pathway and also has pivotal role in intracellular regulation. Monoclonal anti-ubiquitin antibody can be used in immunoblotting and ELISA to localize and detect ubiquitin. Mouse anti-ubiquitin antibody reacts specifically with ubiquitin in mouse, human, bovine and rat.

Immunogen

purified bovine ubiquitin conjugated to KLH.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Monoclonal anti-ubiquitin antibody can be used in western blot to visualize and evaluate ubiquitylated proteins.It can also be used in microarray and immunoblotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Dieser Artikel
U5379SAB1306582SAB5700792
clone

6C1, monoclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

human, rat, mouse, bovine

species reactivity

prokaryotes, eukaryotes

species reactivity

human

species reactivity

rat, human, mouse

antibody form

ascites fluid

antibody form

whole antiserum

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

Gene Information

human ... UBB(7314), UBC(7316)
mouse ... Ubb(22187), Ubc(22190)
rat ... Ubb(192255), Ubc(50522)

Gene Information

human ... UBB(7314), UBC(7316)
mouse ... Ubb(22187), Ubc(22190)
rat ... Ubb(192255), Ubc(50522)

Gene Information

human ... UBC(7316)

Gene Information

human ... UBC(7316)

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

mouse

biological source

rabbit

biological source

rabbit

biological source

rabbit


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Lagerklasse

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable



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Clinton Yu et al.
Nature communications, 6, 10053-10053 (2015-12-04)
The full enzymatic activity of the cullin-RING ubiquitin ligases (CRLs) requires a ubiquitin-like protein (that is, Nedd8) modification. By deamidating Gln40 of Nedd8 to glutamate (Q40E), the bacterial cycle-inhibiting factor (Cif) family is able to inhibit CRL E3 activities, thereby
Andrej Susor et al.
Reproduction (Cambridge, England), 134(4), 559-568 (2007-09-25)
In this study, we performed proteomic analysis of porcine oocytes during in vitro maturation. Comparison of oocytes at the initial and final stages of meiotic division characterized candidate proteins that were differentially synthesized during in vitro maturation. While the biosynthesis
Alexander Espinosa et al.
The Journal of biological chemistry, 286(42), 36478-36491 (2011-08-25)
Ro52 (TRIM21) is an E3 ligase of the tripartite motif family that negatively regulates proinflammatory cytokine production by ubiquitinating transcription factors of the interferon regulatory factor family. Autoantibodies to Ro52 are present in patients with lupus and Sjögren's syndrome, but



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