S2015

SSPE Buffer 20× Concentrate

for Northern and Southern blotting, solution

• Synonym(e): 20X SSPE Buffer, SSPE hybridization buffer, Saline-sodium phosphate-EDTA buffer
• NACRES: NA.25
• UNSPSC Code: 41105319

Eigenschaften

• grade: Molecular Biology
• Quality Segment: 200
• sterility: 0.2 μm filtered
• form: solution
• foreign activity: DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected
• storage temp.: room temp

Beschreibung

General description

SSPE Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. This liquid can be used straight or diluted as needed.

Application

Suitable for:

• Hybridization buffer for Northern and Southern blots
• Washing buffer for microarrays

Features and Benefits

• Convenient ready-to-use liquid
• Multi-purpose use in hybridizations

Preparation Note

SSPE Buffer 20x contains 0.02M EDTA and 2.98M NaCl in 0.2M phosphate buffer (pH 7.4).

Disclaimer

White precipitate/cloudiness may occur after cold shipping/storage due to salt crystallization (normal); re-dissolve by warming at 37 °C for 1–2 h with occasional swirling and use once clear.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable