P8458

Anti-Protein Kinase Cε antibody produced in rabbit

whole antiserum

• Synonym(e): Anti-PKC ε
• UNSPSC Code: 12352203
• NACRES: NA.44
• MDL number: MFCD00162773
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ARR, DB, WB
• Citations: 11

Eigenschaften

• biological source: rabbit
• conjugate: unconjugated
• antibody form: whole antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• mol wt: antigen 90 kDa
• contains: 15 mM sodium azide
• species reactivity: rat
• technique(s): dot blot: 1:50,000, microarray: suitable, western blot: 1:15,000 using rat brain extract
• UniProt accession no.: P09216
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: rat ... Prkce(29340)

Beschreibung

General description

Protein Kinase C (PKC) is a family of serine/threonine (Ser/Thr)-specific protein kinases. Protein kinase Cε (PKCε) is a member of the novel subgroup. The PKCε isoenzyme appears to be widely expressed in rat brain, lung, skin, liver, spleen, thymus, embryonic rat fibroblasts and in low levels, in certain mouse fibroblasts.

Immunogen

synthetic peptide, corresponding to the C-terminal variable (V5) region (amino acids 726-737) of PKC ε.

Application

Anti-Protein Kinase C epsilon antibody may be used:

• for detection of PKCepsilon in whole cell or membrane extracts of HUVEC cells when stimulated with PMA
• in indirectdot blot
• for protein microarray

Anti-Protein Kinase Cε antibody produced in rabbit may be used in:

• western blot
• immunoprecipitation
• enzyme linked immunosorbent assay (ELISA)
• immunohistochemistry
• chemiluminescence detection systems

Biochem/physiol Actions

Anti-Protein Kinase C ε (PKC ε) reacts in immuno-blotting with PKC ε (90 kDa polypeptide) from rat brain extract

Protein Kinase C perform key functions in numerous signalling pathways, in biological systems, through their various isoforms. The PKC isoforms are activated by phosphatidylserine, calcium or phorbol esters. Proteolysis of PKC ^{in vivo} is thought to be mediated by calpains I and II. Multiple functions such as, cellular and vascular regulations, angiogenesis, cell growth, apoptosis, changes in basement membrane thickness, extracellular matrix organisation, MAPK signalling, are attributed to PKC isoforms. PKCepsilon and PKCδ have sometimes opposite effects. PKCepsilon is most important in stimulation of cell growth and differentiation and modulation of cytoskeleton These varied functions implicate PKC isoforms in cardiac hypertrophies and diabetic nephropathy and cardiovascular complications.

Protein kinase Cε (PKC epsilon) is expressed in low levels in several normal murine tissues with the exception of brain but is expressed at high levels in several hemopoietic cell lines and tumors, suggesting its possible role in tumorigenesis. Overexpression and stimulation of PKC ε causes an increased growth rate and higher cell densities in monolayers cultures.

Physical form

Rabbit Anti-Protein Kinase C ε is supplied as a liquid containing 0.1% sodium azide as preservative.

Preparation Note

For continuous use, store at 2–8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.