Additionally, an extensive literature search revealed an article discussing the effect of type IV collagen on fibrin assembly kinetics. The authors indicated that as the concentration of collagen increased from 0 to 100 ng/ml, the rate of fibrin assembly increased, as evidenced by an increase in the initial slope seen in Fig. 1. The article can be found at https://www.jbc.org/content/263/15/7043.full.pdf. It may be helpful to use similar guidelines.
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M7403
MCDB 153 Medium
With trace elements, L-glutamine and 28mM HEPES, without sodium bicarbonate, powder, suitable for cell culture
Synonym(e):
Molecular, Cellular, and Development Biology 153 medium
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|
Über diesen Artikel
NACRES:
NA.75
UNSPSC Code:
12352207
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
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Unterstützung erhaltenQuality Segment
form
powder
technique(s)
cell culture | mammalian: suitable
components
NaHCO3: no
L-glutamine: 0.8772 g/L
glucose: 1.081 g/L (Dextro)
sodium pyruvate: 0.055 g/L
phenol red: 0.001242 g/L
HEPES: 6.6 g/L (25mM)
shipped in
ambient
storage temp.
2-8°C
General description
MCDB 153 media are modifications of Ham′s nutrient mixture F-12, designed for the growth of human keratinocytes, clonal growth of chicken embryo fibroblasts, and Chinese hamster ovary (CHO) cells using low levels of fetal bovine serum protein (FBSP), extensive trace elements or no serum protein.
Application
MCDB 153 Medium has been used as a component of melanoma isolation media (MIM) to culture human non-metastatic and metastatic melanoma cells.
Preparation Note
Formulated to contain 17.6 grams of powder per liter of medium.
Supplement with 1.18 g/L sodium bicarbonate.
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Dieser Artikel | |||
|---|---|---|---|
| form powder | form liquid | form powder | form powder |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| components NaHCO3: no | components L-glutamine: no | components sodium pyruvate: 0.11 g/L | components HEPES: 7.149 g/L (30mM) |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| Quality Level 500 | Quality Level 400 | Quality Level - | Quality Level - |
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Lagerklasse
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Verwandter Inhalt
Instructions
Hans Peter Weitzenböck et al.
Cancer medicine, 11(4), 956-967 (2021-12-25)
Malignant melanoma is the deadliest form of skin cancer and NRF2 has been proposed as a main regulator of tumor cell malignancy. Still the mechanisms how NRF2 is contributing to melanoma progression are incompletely understood. Here we analyzed the effects
Andrew S McNeal et al.
eLife, 10 (2021-11-24)
Benign melanocytic nevi frequently emerge when an acquired BRAFV600E mutation triggers unchecked proliferation and subsequent arrest in melanocytes. Recent observations have challenged the role of oncogene-induced senescence in melanocytic nevus formation, necessitating investigations into alternative mechanisms for the establishment and
Matthew L Fisher et al.
Molecular carcinogenesis, 55(12), 2024-2036 (2015-12-24)
Melanoma is a metastatic cancer associated with poor survival. Here, we study a subpopulation of melanoma cancer cells displaying melanoma cancer stem cell (MCS cells) properties including elevated expression of stem cell markers, increased ability to survive as spheroids, and
Global Trade Item Number
| SKU | GTIN |
|---|---|
| M7403-10X1L | 04061834061991 |
| M7403-1L | 04061834062004 |
| M7403-10L | 04061834061984 |
| M7403-BIBC | 04061826573938 |
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What is the recommended protocol for co-culturing keratinocytes, fibroblasts, and endothelial cells using MCDB153 media?
What is the recommended protocol for co-culturing keratinocytes, fibroblasts, and endothelial cells using MCDB153 media?
1 answer-
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Unless otherwise stated, this product is intended for research use only and is not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses, or any type of consumption or application to humans or animals.
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