M3320

Monoclonal Anti-MBNL1 antibody produced in mouse

~1 mg/mL, clone HL 1822 (3A4-1E9), purified immunoglobulin, buffered aqueous solution

• Synonym(e): Anti-CUG Triplet Repeat Expansion Double-stranded RNA-binding protein, Anti-EXP, Anti-KIAA0428, Anti-Muscleblind-like protein, Anti-Triplet-expansion RNA-binding protein
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD09039233
• Conjugate: unconjugated
• Clone: HL 1822 (3A4-1E9), monoclonal
• Application: ARR, ICC, IHC, WB
• Citations: 8

Eigenschaften

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: HL 1822 (3A4-1E9), monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~42 kDa
• species reactivity: monkey, mouse, human
• concentration: ~1 mg/mL
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, microarray: suitable, western blot: 0.2-0.5 μg/mL using HeLa nuclear cell extract
• isotype: IgG1
• UniProt accession no.: Q9NR56
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MBNL1(4154) ; mouse ... Mbnl1(56758)

Beschreibung

General description

Muscle blind-like 1 (MBNL1) is a homolog of human muscle blind like proteins. Proteins of the MBNL (muscleblind-like) family are mainly expressed in skeletal muscle neuronal tissues, thymus, liver and kidney.

Immunogen

recombinant muscleblind-like1 (MBNL1) fusion protein.

Application

Monoclonal Anti-MBNL1 antibody produced in mouse has been used in: immunoblotting,[1] immunohistochemistry and immunocytochemistry

Biochem/physiol Actions

Proteins of the MBNL (muscleblind-like) family enhance inclusion or exclusion of specific exons on different pre-mRNAs by inhibiting the activity of CUG-BP and ETR-3-like factors (CELF proteins) bound to distinct intronic sites. MBNL1 may be implicated in the stimulation of muscle differentiation. Mice knockouts for the MBNL1 gene develop muscle, eye, and RNA splicing abnormalities that are characteristic of the neuromuscular disease myotonic dystrophy (DM) disease.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)