F0552

Fas Ligand from mouse

>95% (SDS-PAGE), recombinant, expressed in mouse NSO cells, lyophilized powder

• MDL-Nummer: MFCD00285792
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Rekombinant: expressed in mouse NSO cells
• Qualitätsniveau: 100
• Assay: >95% (SDS-PAGE)
• Form: lyophilized powder
• Mol-Gew.: monomer calculated mol wt ~18 kDa; 28-32 kDa by SDS-PAGE
• Verunreinigungen: endotoxin, tested
• UniProt-Hinterlegungsnummer: P41047
• Lagertemp.: −20°C
• Angaben zum Gen: mouse ... Fasl(14103)

Beschreibung

Allgemeine Beschreibung

FASLG (Fas ligand) acts as a ligand for Fas receptor, and is a major protein involved in programmed cell death, apoptosis. Soluble Fas (sFAS) is usually detected in plasma prior to apoptosis.[1]

Anwendung

Fas Ligand (FASLG) from mouse has been used for-

• the induction of apoptosis in PC12 cells and
• the induction of migration in BV-2 murine microglial cells.[2][3]

Biochem./physiol. Wirkung

FASLG (Fas ligand) and Fas receptor constitute the basic elements in apoptosis. Interaction of FASLG with Fas receptor leads to activation of caspase-8. This caspase in turn leads to activation of effector caspases such as caspase-3, -6 and -7. This cascade results in the hydrolysis of nuclear and cytoplasmic components. Expression of FASLG is induced by nuclear factor-κB (NFκB). NFκB/FASLG pathway facilitates the suppression of p,p′-DDT (dichlorodiphenoxytrichloroethane)-induced cell toxicity by vitamin C and E.[4] In CD4⁺ T cells, this protein is expressed on stimulus by T-cell receptor (TCR), both during normal and pathological conditions, such as alcohol exposure.[5]

Fas ligand, a protein belonging to the tumor necrosis factor (TNF) family of cytokines, induces apoptosis in cells expressing the cell membrane receptor Fas (CD95/Apo-1).

Protein belonging to the tumor necrosis factor (TNF) family of cytokines; induces apoptosis in cells expressing the cell membrane receptor Fas (CD95/Apo-1).

Sonstige Hinweise

Mouse Fas Ligand, N-terminal 6X histidine-tagged, encodes amino acid residues 132-279.

Physikalische Form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Hinweis zur Analyse

Measured by its ability to induce apoptosis in Jurkat cells.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable