Fortfahren mit
E0632
Enterokinase from porcine intestine
≥0.5 units/mg solid
Synonym(e):
Enteropeptidase
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 1500 units | Warenkorb auf Verfügbarkeit prüfen | CHF 365.00 |
Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-761-1
NACRES:
NA.54
MDL number:
Specific activity:
≥0.5 units/mg solid
CHF 365.00
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
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Unterstützung erhaltenform
salt-free, lyophilized powder
Quality Level
specific activity
≥0.5 units/mg solid
mol wt
150 kDa
storage temp.
−20°C
Application
Enterokinase from porcine intestine has been used in a study to report a new experimental model of the anomalous pancreatico-biliary junction. Enterokinase from porcine intestine has also been used in a study to investigate the insulinotropic region of the gastric inhibitory polypeptide.
The enzyme from Sigma has been used for the activation of trypsinogen in order to measure the activity of trypsin in hog pancreas. The study showed that antimicrobial treatment reduces intestinal microflora and improves protein digestive capacity without changes in villous structure of weanling pigs.[1]
Biochem/physiol Actions
Enterokinase is a membrane bound serine protease that specifically and rapidly converts trypsinogen to trypsin, thereby, triggering the conversion of other zymogens to active enzymes. It has a molecular mass of approximately 150 kDa. The enzyme is a heterodimer consisting of 35-47 kDa subunits. The light and the heavy chains are linked by two disulfide bridges. It is a glycoprotein containing 35% carbohydrate. The polypeptide chain of trypsinogen is hydrolyzed only after an -(Asp)4-Lys- sequence. The enzyme is inhibited by soybean trypsin inhibitor. Enterokinase is typically used in protein modification and amino acid sequence determination.
Other Notes
One unit will produce 1.0 nanomole of trypsin from trypsinogen per min at pH 5.6 at 25 °C.
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| specific activity ≥0.5 units/mg solid | specific activity ≥100 units/mg protein | specific activity - | specific activity 300-1,500 units/mg protein |
| form salt-free, lyophilized powder | form lyophilized powder | form powder | form salt-free, lyophilized powder |
| mol wt 150 kDa | mol wt 150 kDa | mol wt 150 kDa (consisting of 115kDa and 35kDa subunits.) | mol wt - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Verwandter Inhalt
QC Methods
Werner Hartwig et al.
Surgery, 144(3), 394-403 (2008-08-19)
A noninvasive model of necrohemorrhagic pancreatitis induced by simultaneous intravenous cerulein/enterokinase (EK) infusion has recently been established in rats. The aim of the present study was to establish this new model in mice and to compare it with the rat
T Benhidjeb et al.
Journal of pediatric surgery, 31(12), 1670-1674 (1996-12-01)
A model of anomalous pancreatico-biliary junction was developed and used to investigate a possible role in the development of choledochal cyst and tumors of the biliary tract. An anastomosis was constructed between an isolated pancreas-duodenal segment and the gallbladder in
Complementary DNA cloning and sequencing of rat enteropeptidase and tissue distribution of its mRNA.
N Yahagi et al.
Biochemical and biophysical research communications, 219(3), 806-812 (1996-02-27)
A cDNA clone encoding enteropeptidase (EC 3.4.21.9), a key enzyme for the conversion of trypsinogen to trypsin, was isolated from a rat duodenal mucosa cDNA library. Sequences of the 3585 base pair clone predicted that enteropeptidase is synthesized as a
Global Trade Item Number
| SKU | GTIN |
|---|---|
| E0632-1.5KU | 04061833601181 |