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B5283

Sigma-Aldrich

Monoklonaler Anti-Kaninchen-IgG-Antikörper (γ-kettenspezifisch)

clone RG-96, purified from hybridoma cell culture

Synonym(e):

Monoclonal Anti-Rabbit IgG (γ-chain specific)

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.46

Biologische Quelle

mouse

Konjugat

biotin conjugate

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

secondary antibodies

Klon

RG-96, monoclonal

Form

buffered aqueous solution

Speziesreaktivität

rabbit

Darf nicht reagieren mit

goat, feline, pig, guinea pig, rat, bovine, canine, human, horse, sheep, chicken

Methode(n)

direct ELISA: 1:150,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000
western blot (chemiluminescent): 1:200,000-1:400,000

Isotyp

IgG1

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

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Allgemeine Beschreibung

Das Produkt erkennt ein Epitop, das sich an der γ-(Schwer-)Kette von Kaninchen-IgG befindet. Beim Immunblotting erkennt der Antikörper sowohl native als auch denaturierte Formen von Kaninchen-IgG. In ELISA ist der Antikörper spezifisch für Kaninchen-IgG und zeigt keine Kreuzreaktivität mit Kaninchen-IgA und -IgM oder Human-IgG, -IgA und -IgM. Es wurde keine Kreuzreaktion mit IgG von folgenden Spezies festgestellt: Rind, Katze, Huhn, Hund, Ziege, Meerschweinchen, Pferd, Schwein, Ratte oder Schaf.
IgG (immunoglobulin G) antibody is secreted by B cells and is found in blood and extracellular fluids and is enriched in mother′s milk. The variable region of IgG antibody is specific to antigens and is highly conserved.

Anwendung

Monoclonal Anti-Rabbit IgG (γ-chain specific)-Biotin is suitable for ELISA at a working dilution of 1:150,000. It may be used for immunohistochemistry of formalin-fixed, paraffin-embedded sections at a dilution of 1:1000. The antibody was used in β-catenin ELISA at a dilution of 1:2000.
Anti-Rabbit IgG (γ-chain specific)-Biotin antibody, Mouse monoclonal has been used in β-Catenin enzyme immunoassay. It has also been used in immunohistochemistry for examining histology of mammary glands.

Biochem./physiol. Wirkung

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to monoclonal Anti-Rabbit IgG (γ-chain specific) antibody allows for the binding of various labels such as avidin or streptavidin. IgG antibody has its function similar to IgM antibody in complement system activation.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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