B4554

BES

BioPerformance Certified, suitable for cell culture, ≥99.0%

• Synonym(e): N,N-Bis-(2-hydroxyethyl)-2-amino-ethansulfonsäure, N,N-Bis-(2-hydroxyethyl)-taurin
• Empirische Formel (Hill-System): C₆H₁₅NO₅S
• CAS-Nummer: 10191-18-1
• Molekulargewicht: 213.25
• UNSPSC Code: 12161700
• NACRES: NA.25
• PubChem Substance ID: 24891788
• EC Number: 233-465-5
• Beilstein/REAXYS Number: 1781572
• MDL number: MFCD00007533

Eigenschaften

• grade: BioPerformance Certified
• assay: ≥99.0%
• form: crystalline
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin and bioburden, tested
• pH: 2.5-5.0 (25 °C, 213.3 g/L)
• useful pH range: 6.4-7.8
• pKa (25 °C): 7.1
• solubility: water: 0.5 g/mL, clear, colorless
• application(s): diagnostic assay manufacturing
• foreign activity: DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected
• storage temp.: room temp
• SMILES string: OCCN(CCO)CCS(O)(=O)=O
• InChI: 1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12)
• InChI key: AJTVSSFTXWNIRG-UHFFFAOYSA-N

Beschreibung

General description

N-N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid (BES) is a zwitterionic sodium sulphate buffer used in biochemistry and molecular biology research. BES buffer used as a secondary pH standard can help maintain a stable pH range in solutions, particularly in the range of 6.2-7.6 pH range of 6.6 to 7.4. It ​is used in a study to evaluate that buffers minimize the action of interfering factors in the bacterial endotoxins test. The application of BES as a buffer solution for calcium phosphate-mediated transfection of eukaryotic cells with plasmid DNA has also been studied.

Application

BES buffer has been used:

• as a supplement in opti-minimal essential medium (MEM) culture media to culture thymocytes for In vitro analysis of thymocyte apoptosis[1]
• as a component of borate buffer saline (BBS) for adeno-associated virus (AAV) vector production, extraction, and purification
• as a component of diluent one for semen collection and cryopreservation[2]

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves