B3428

Anti-Bax antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(e): Anti-BCL2L4
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00282585
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ARR, WB
• Citations: 29

Eigenschaften

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 23 kDa
• species reactivity: human, rat, mouse
• technique(s): microarray: suitable, western blot: 1:2,000 using whole cell extract of MCF7 human breast adenocarcinoma cell line
• UniProt accession no.: Q07812
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... BAX(581) ; mouse ... Bax(12028) ; rat ... Bax(24887)

Beschreibung

General description

Bax is a 21kD integral organelle membrane protein that belongs to the Bcl-2 family and is expressed mainly in the mitochondria. It exist in three cytosolic forms Bax β (24 kDa), Bax γ (5 kDa) and Bax δ (16 kDa) generated by alternative splicing.

Immunogen

synthetic peptide GGPTSSEQIMKTGALLLQGF-K corres- ponding to the N-terminal region of Bax of human origin (Bax α, amino acids 11-30 with C-terminally added lysine), conjugated to KLH as immunogen. This sequence is identical in human Bax β and highly conserved in rat and mouse Bax α (single amino acid substitution) and human Bax.

Application

Anti-BMPR2 (836-850) antibody produced in rabbit is suitable for western blotting at a concentration of 1:500-1:2,000.

Biochem/physiol Actions

BAX is a proapoptotic protein that forms homodimer to accelerate apoptotic death whereas its heterodimerization with Bcl-2 or Bcl-xL can block apoptosis^. BAX binds to the mitochondrial permeability transition pore complex (PTPC), and when expressed in cells, it induces mitochondrial dysfunction and the release of cytochrome C, leading to activation of caspases and triggers apoptosis. Loss-of-function mutation in the BAX gene results in Hematopoietic malignancies.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable