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A9294

Anti-Goat IgG (whole molecule)−Agarose antibody produced in rabbit

IgG fraction of antiserum, PBS suspension

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Über diesen Artikel

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
Conjugate:
agarose conjugate
Clone:
polyclonal
Application:
IEP
Citations:
5


biological source

rabbit

Quality Level

conjugate

agarose conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

PBS suspension

species reactivity

goat

extent of labeling

~2.4 mg Ab per mL

technique(s)

immunoelectrophoresis: suitable

matrix

Cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix spacer

1 atom

capacity

1 mg/mL, resin binding capacity (goat IgG)

storage temp.

2-8°C

target post-translational modification

unmodified

General description

An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It mainly helps in immune defense. . IgG, a glycoprotein, is the major class of immunoglobulin. Goat IgG has two subclasses- IgG1 and IgG2.
IgGs are glycoprotein antibodies that modulate several immune responses. Goat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-goat IgGs are useful tools for the analysis of target proteins.

Immunogen

pooled normal goat serum

Application

Anti-Goat IgG (whole molecule) Agarose antibody produced in rabbit has been used in protein purification, immunoprecipitation/chromatin immunoprecipitation assay.
c-RET was immunoprecipitated from overexpressing MDCK cells using agarose-linked rabbit anti-goat IgG. Homogenates were incubated with conjugated beads for 2 hours on ice.

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.
Rabbit Anti-Goat IgG (whole molecule)-Agarose antibody binds to goat IgG.

Physical form

Suspension in phosphate buffered saline containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Dieser Artikel
A3316G7277A5420
conjugate

agarose conjugate

conjugate

agarose conjugate

conjugate

gold conjugate

conjugate

peroxidase conjugate

species reactivity

goat

species reactivity

human

species reactivity

-

species reactivity

goat

technique(s)

immunoelectrophoresis: suitable

technique(s)

Ouchterlony double diffusion: suitable

technique(s)

dot blot: suitable, western blot: suitable

technique(s)

direct ELISA: 1:40,000, dot blot: 1:80,000-1:160,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

biological source

rabbit

biological source

goat

biological source

goat

biological source

rabbit

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C


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Lagerklasse

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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Verwandter Inhalt


Expression of a translationally fused TAP-tagged plasma membrane proton pump in Arabidopsis thaliana
Rodrigues RB, et al.
Biochemistry, 53(3) (2014)
Specific IgG for cat allergens in patients with allergic conjunctivitis
Miyama A, et al.
International Ophthalmology, 575-586 (2015)
An essential role for Orc6 in DNA replication through maintenance of pre-replicative complexes
Semple JW, et al.
The Embo Journal, 25(21), 5150-5158 (2006)



Global Trade Item Number

SKUGTIN
A9294-2ML04061838168788

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