94149

Atto 647N-Streptavidin

BioReagent, suitable for fluorescence

• Synonym(e): Streptavidin-Atto 647N
• NACRES: NA.25
• UNSPSC Code: 12161900

Eigenschaften

• product line: BioReagent
• Quality Segment: 100
• form: powder
• manufacturer/tradename: ATTO-TEC GmbH
• λ: in ethanol (with 0.1% trifluoroacetic acid)
• UV absorption: λ: 650.0-656.0 nm A_max
• suitability: corresponds for gel electrophoresis, suitable for fluorescence
• storage temp.: −20°C

Beschreibung

General description

Atto 647N is a new generation fluorescent dye dedicated towards the red spectral region. It is a cationic dye and carries a net electrical charge of +1 post substrate coupling reaction. Streptavidin, isolated from Streptomyces avidinii, is a tetrameric protein of 4 x 13.2 kDa which binds very tightly to the small molecule biotin. The dissociation constant of the complex is extremely small (Kd ≈ 10-15 M), ranking among the strongest non-covalent interactions. Atto streptavidin conjugates are readily soluble in water.

Application

Die Atto Fluoreszenzfarbstoffe wurden für die hochsensitive Anwendung bis hin zur Einzel-Molekül-Detektion (SMD) entwickelt. Sie haben eine starre Molekülstruktur, was das Auftreten einer cis-trans-Isomerisierung unterbindet. Daher besitzen diese Farbstoffe eine ausserordentlich hohe Fluoreszenzintensität mit einer geringen spektralen Verschiebung nach der Kopplung an Biomoleküle.

Features and Benefits

Atto 647N- Streptavidin has the following benefits-

• Highly photostable. Strong absorption.
• Excellent fluorescent quantum yield.
• Highly resistant to ozone degradation.
• Minimal triplet formation.

Analysis Note

dye-to-protein ratio ≥1.5

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)