83330

Pyruvat-Kinase aus Kaninchenmuskel

crystalline suspension in 3.8 M ammonium sulfate solution, ~350 U/mg protein (10-40 mg/ml)

• Synonym(e): ATP:pyruvate 2-O-phosphotransferase, PK, Phospho-enolpyruvat-Kinase
• CAS-Nummer: 9001-59-6
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• EC Number: 232-616-2
• NACRES: NA.54
• MDL number: MFCD00081946
• EG-Nummer: 2.7.1.40 (BRENDA, IUBMB)
• Specific activity: ~350 U/mg protein (10-40 mg/ml)

Eigenschaften

• form: suspension
• quality: crystalline suspension in 3.8 M ammonium sulfate solution
• specific activity: ~350 U/mg protein (10-40 mg/ml)
• mol wt: M_r ~230000
• pH: ~6
• foreign activity: lactate dehydrogenase ≤0.01%
• storage temp.: 2-8°C

Beschreibung

Biochem/physiol Actions

Molecular Weight: 237 kDa and exists as a tetramer of four equal subunits of molecular weight 57 kDa.
Isoelectric Point: 7.6
Optimal pH: ∼7.5
Optimal Temperature: 25°C
ΕA₂₈₀ = 0.54 for 1 mg(p)/ml, 1 cm path
Reported K_M values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.

Pyruvate kinase catalyzes the conversion of phosphoenolpyruvate to pyruvate, whitch is the rate-limiting final step in glycolysis.

Other Notes

1 U entspricht der Enzymmenge, die 1 μmol PEP pro Minute bei pH 7.4 und 25°C umsetzt

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)