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79189

Abberior® FLIP 565, NHS-ester

for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)

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UNSPSC Code:
12352200


Quality Segment

form

solid

concentration

≥60.0% (degree of coupling)

solubility

DMF: 1 mg/mL, clear

fluorescence

λem 580 nm±5 nm in PBS, pH 7.4

storage temp.

−20°C

General description

Absorption Maximum (off-state) λmax:314 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 47,000 M-1cm-1 (MeOH)
Fluorescence Maximum, λfl:580 nm (PBS, pH 7.4)
Photoactication Wavelength: 310-380 (one-photon activation)
650-800 (two-photon activation)
Fluorescence Quantum Yield, η: 0.38 (PBS, pH 7.4)

Application

Abberior® FLIP 565 conjugated with secondary antibody has been used for STORM (stochastic optical reconstruction microscopy) imaging of COS-7 and S180 cells.[1][2]
Designed and tested for fluorescent super-resolution microscopy

Legal Information

abberior is a registered trademark of Abberior GmbH


Lagerklasse

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Verwandter Inhalt

79189, Abberior FLIP 565 NHS ester


Remi Galland et al.
Nature methods, 12(7), 641-644 (2015-05-12)
Single-objective selective-plane illumination microscopy (soSPIM) is achieved with micromirrored cavities combined with a laser beam-steering unit installed on a standard inverted microscope. The illumination and detection are done through the same objective. soSPIM can be used with standard sample preparations
Nicolas Olivier et al.
Biomedical optics express, 4(6), 885-899 (2013-06-14)
3D STORM is one of the leading methods for super-resolution imaging, with resolution down to 10 nm in the lateral direction, and 30-50 nm in the axial direction. However, there is one important requirement to perform this type of imaging:
Marcus Dyba et al.
Nature biotechnology, 21(11), 1303-1304 (2003-10-21)
We report immunofluorescence imaging with a spatial resolution well beyond the diffraction limit. An axial resolution of approximately 50 nm, corresponding to 1/16 of the irradiation wavelength of 793 nm, is achieved by stimulated emission depletion through opposing lenses. We



Global Trade Item Number

SKUGTIN
E7887-100MG04061833608548

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