MABF2003

Anti-CD11a Antibody, clone BU17

clone BU17, from mouse

• Synonym(e): Integrin alpha-L, CD11 antigen-like family member A, Leukocyte adhesion glycoprotein LFA-1 alpha chain, LFA-1A, Leukocyte function-associated molecule 1 alpha chain
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: BU17, monoclonal
• Application: FACS
• Citations: -

Eigenschaften

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: BU17, monoclonal
• species reactivity: human
• packaging: antibody small pack of 25 μg
• technique(s): flow cytometry: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_002200.2
• UniProt accession no.: P20701
• target post-translational modification: unmodified
• Gene Information: human ... ITGAL(3683)

Beschreibung

General description

128.77 kDa calculated.

Integrin alpha-L (UniProt: P20701; also known as CD11 antigen-like family member A, Leukocyte adhesion glycoprotein LFA-1 alpha chain, LFA-1A, Leukocyte function-associated molecule 1 alpha chain, CD11a) is encoded by the ITGAL (also known as CD11A) gene (Gene ID: 3683) in human. Integrins are part of a family of heterodimeric transmembrane glycoproteins that recognize a variety of ligands and control numerous physiologic functions in cells. CD11a is a single-pass type I membrane protein that noncovalently associates with beta2 integrin to forms a heterodimer and serve as a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils, and eosinophils. It is also involved in generation of common lymphoid progenitor cells in bone marrow. In resting T-cells, about 40% of CD11a is constitutively phosphorylated and phosphorylation causes conformational changes needed for ligand binding and is considered to be essential for activation by some physiological agents. CD11a is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off in the mature form. It has a large extracellular domain (aa 26-1090), a transmembrane domain (aa 1091-1111), and a cytoplasmic domain 1112-1170). Three isoforms of CD11a have been described that are produced by alternative splicing.

Immunogen

Epitope: extracellular domain

Waldenstrom macroglobulinemia lymphoplasmacytic cells.

Application

Anti-CD11a, clone BU17, Cat. No. MABF2003, is a mouse monoclonal antibody that detects CD11a and has been tested for use in Flow Cytometry.

Flow Cytometry Analysis: A representative lot detected CD11a in Flow Cytometry applications (Baker, M.P., et. al. (1998). Blood. 92(8):2830-43).

Research Category
Inflammation & Immunology

Biochem/physiol Actions

Clone BU17 detects CD11a in human cells. It targets an epitope with in the extracellular domain.

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Flow Cytometry in one million human peripheral blood mononuclear cells.

Flow Cytometry Analysis: 1 µg of this antibody detected CD11a in one million human peripheral blood mononuclear cells.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.