IM40

Anti-MMP-7 (Ab-1) Mouse mAb (141-7B2)

liquid, clone 141-7B2, Calbiochem^®

• Synonym(e): Anti-Matrilysin, Anti-Matrix Metalloproteinase 7, Anti-PUMP-1
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 141-7B2, monoclonal
• Form: liquid
• Enthält: ≤0.1% sodium azide as preservative
• Speziesreaktivität: human
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• Isotyp: IgG1
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... MMP7(4316)

Beschreibung

Allgemeine Beschreibung

Purified mouse monoclonal antibody. Recognizes the ~28 kDa latent form of MMP-7.

Recognizes the ~28 kDa latent form of MMP-7 in TPA-treated SW620 cells. Does not recognize the active form of MMP-7.

This Anti-MMP-7 (Ab-1) Mouse mAb (141-7B2) is validated for use in Immunoblotting, Paraffin Sections for the detection of MMP-7 (Ab-1).

Immunogen

Human

human rectal carcinoma MMP-7

Anwendung

Immunoblotting (5-10 g/ml, see application references)
Paraffin Sections (see application references)

Verpackung

Please refer to vial label for lot-specific concentration.

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.

Rekonstituierung

Following initial thaw, aliquot and freeze (-20°C).

Hinweis zur Analyse

Positive Control
CaR-1 or TPA treated SW620 cells

Sonstige Hinweise

Brunner, K.L, et al. 1995. Proc. Natl. Acad. Sci. USA92, 7362.
Imai, K., et al. 1995. J. Biol. Chem.270, 6691.
Lichtinghagen, R., et al. 1995. Eur. J. Clin. Chem. Clin. Biochem.33, 65.
Nakano, A., et al. 1995. J. Neurosurg.83, 298.
Takino, T., et al. 1995. J. Biol. Chem.270, 23013.
Woessner, J.F. 1991. FASEB J.5, 2145.
Yamamoto, H., et al. 1995. J. Clin. Lab. Anal.9, 297.
Gaire, M., et al. 1994. J. Biol. Chem.269, 2032.
McDonnell, S., et al. 1994. Biochem. Soc. Trans.22, 58.
Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol.2, 861.
Stetler-Stevenson, W.G., et al. 1993. FASEB J.7, 1434.
Woessner, J.F. 1991. FASEB J.5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Sem. Cancer Biol., ed. M. M. Gottesman. Vol. 1(2), 99.
Woessner, J.F., Jr and Taplin, C.J. 1988. J. Biol. Chem.263, 16918.
Sellers, A. and Woessner, J.F., Jr. 1980. Biochem. J.189, 521.

Does not recognize the ~19 kDa active form of MMP-7. To detect active MMP-7, use Cat. No. IM47L. Does not cross-react with human MMP-1, MMP-2, MMP-3, MMP-8, MMP-9 or MMP-13. For optimal detection, SW620 cells should be treated with TPA (100 ng/ml) to induce expression of MMP-7. Antibody should be titrated for optimal results in individual systems.

Rechtliche Hinweise

Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable