This product is not charcoal treated. The datasheet is a general document for BSA products.
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|
Über diesen Artikel
biological source
bovine
Quality Segment
assay
≥96%
form
lyophilized powder
purified by
cold ethanol fractionation
origin
USA origin
technique(s)
ELISA: suitable, blood typing: suitable, microbiological culture: suitable
impurities
BT and VSV virus, tested
loss
≤5%
pH
5.2
solubility
water: soluble (40 mg/ml)
UniProt accession no.
foreign activity
BT Virus, none detected, VSV Virus, none detected
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
General description
Application
- Rinderserumalbumin wird in einer Lösung zur Isolierung von Herzmitochondrien verwendet.
- Es wird in Puffer für den Zytotoxizitätsassay verwendet.
- Es wird zur Einleitung einer Nephropathie durch Proteinüberlastung bei Ratten eingesetzt.
Biochem/physiol Actions
Preparation Note
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| assay ≥96% | assay ≥96% | assay ≥98% (agarose gel electrophoresis) | assay ≥98% |
| origin USA origin | origin USA origin | origin USA origin | origin USA origin |
| technique(s) ELISA: suitable, microbiological culture: suitable, blood typing: suitable | technique(s) blood typing: suitable, microbiological culture: suitable | technique(s) cell culture | mammalian: suitable | technique(s) immunocytochemistry: suitable |
| biological source bovine | biological source bovine | biological source bovine | biological source bovine |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
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Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protokolle
Spectrophotometric assay at 260 nm measures nuclease S1 activity, vital for nucleic acid research, with defined enzyme unit criteria.
Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
Artikel
Explore serum albumin's role in serum-free cell culture systems for biomanufacturing.
Verwandter Inhalt
Learn about bovine serum albumin (BSA) structure, molecular weight, function, solubility, how BSA is made, purification methods, applications and purchasing.
Discover why blood proteins such as bovine serum albumin, human serum albumin, globulins, and more are crucial for cell culture.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A4503-5KG | 04061833458358 |
| A4503-50G | 04061835553402 |
| A4503-1KG | 04061835553389 |
| A4503-500G | 04061835553396 |
| A4503-10KG | 04061833693216 |
| A4503-100G | 04061835517619 |
| A4503-10G | 04061835504480 |
| A4503-5G | 04061833693223 |
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The data sheet lists several methods for further purification of BSA after ethanol precipitation but does not say which of these were used for A4503. Is A4503 charcoal treated? The data sheet is the same for other BSA powders (e.g., A2153).
1 answer-
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Is this product biotin-free?
1 answer-
This product is not tested for the presence of biotin. However, Albumin products prepared by cold ethanol fractionation are expected to be biotin-free.
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Bonjour y a t-il un risque l'altération du produit si la bovin sérum albumine a été conservée pendant 15 heures en dehors du frigo dans une pièce a température ambiante ?
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Product A4503 (Bovine Serum Albumin) will not spoil following storage for 15 hours at ambient room temperature.
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can A4503 product be used for primary neuron preparation?
1 answer-
This product has not been evaluated for mammalian cell culture. Please see the datasheet for the recommended applications for this product:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/384/276/a4503dat.pdfHelpful?
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Is Product No. A4503, Albumin from bovine serum, tested and found to be endotoxin-free?
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No. If you need such a grade, we would recommend Product No. A8806.
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Do you offer a kit for conjugating ligands to bovine serum albumin (Product A4503)?
1 answer-
Yes. It is our Product No. MBK1.
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Is Product No. A4053, Albumin from bovine serum, sterile? If not, how would recommend sterilizing it?
1 answer-
This product is not necessarily sterile. Solutions of albumin cannot be autoclaved, so in order to sterilize it we would recommend that it be dissolved in the appropriate medium (water, saline or buffered saline), followed by sterile filtration through a low protein-binding membrane.
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Is Product No. A4503, Albumin from bovine serum, tested and found to be protease-free?
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No. If you need such a grade, we would recommend Product No. A3059.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is Product No. A4503, Albumin from bovine serum, of US origin?
1 answer-
It is typically from animals born and raised in the US. You may confirm this information by downloading the Certificate of Origin (CofO) for any lot of A4503 from our website. From the product detail page, simply type in the lot number in the box below the words Certificate of Origin, and click GO.
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