Since certain components in 10X medium concentrates precipitate at pH 7.0, it is necessary to adjust the pH of these solutions to maintain solubility.
Therefore, it may be necessary for the user to adjust the pH of the 1X preparation with sterile 1 N NaOH or 1 N HCl. Procedure For Dilution to 1X (1 Liter):
(NOTE: Dilution of 10X solutions should be performed with sterile containers, components, and equipment.)
• Aseptically measure out approximately 850 ml of tissue culture grade water into an appropriate size container.
• While gently stirring the water, add 100 ml of 10X medium.
• To the solution, add the required amount of sodium bicarbonate ( 0.35 g/L) and L-glutamine (0.1 g/L), at 1X
• While stirring, adjust the solution to desired pH with 1 N NaOH or 1 N HCl.
• Bring medium to final volume with additional tissue culture grade water.
• It is recommended to sterile filter the prepared medium through a 0.22 ?m sterile membrane, or, for example, a Stericup.
• Aseptically dispense into sterile containers. Store refrigerated at 2-8° C
Fortfahren mit
M9163
Medium 199
With Hanks′ salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10X
Synonym(e):
M199 Medium, TCM 199
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Über diesen Artikel
Produktname
Medium 199, 10 ×, With Hanks′ salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
concentration
10 ×
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
phenol red: yes
HEPES: no
sodium pyruvate: no
Hanks’ salts (2% CO2): yes
L-glutamine: no
NaHCO3: no
shipped in
ambient
storage temp.
2-8°C
General description
Preparation Note
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| form liquid | form liquid | form liquid | form powder |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility - |
| components phenol red: yes | components NaHCO3: no | components L-glutamine: no | components L-glutamine: yes |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
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Lagerklasse
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Artikel
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
Verwandter Inhalt
Discover the formulation for Medium 199 for cell culture applications, including vaccine development.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| M9163-6X500ML | 04061834064022 |
| M9163-500ML | 04061834064015 |
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Hi, if I have 10x Medium 199, how can I dilute it in order to have 1x and grow cells?
1 answer-
Helpful?
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