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L5014

Lipopolysaccharides (rough strains) from Escherichia coli J5 (Rc mutant)

Synonym(e):

LPS

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Über diesen Artikel

CAS-Nummer:
93572-42-0
UNSPSC Code:
12352201
EC Number:
297-473-0
NACRES:
NA.25
MDL number:
MFCD00164401
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
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biological source

Escherichia coli (J5 Rc mutant)

Quality Level

200

form

lyophilized powder

impurities

<3% Protein (Lowry)

color

white to faint beige

solubility

water: 0.90-1.10 mg/mL, hazy to turbid, colorless to faintly yellow

shipped in

ambient

storage temp.

2-8°C

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Lipopolysaccharides (rough strains) from Escherichia coli J5 (Rc&nbsp;mutant)

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L5014

Lipopolysaccharides (rough strains) from Escherichia coli J5 (Rc mutant)

Lipopolysaccharides from Escherichia coli O55:B5 &#947;-irradiated, BioXtra, suitable for cell culture

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Lipopolysaccharides from Escherichia coli O55:B5 FITC conjugate

Sigma-Aldrich

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Lipopolysaccharides from Escherichia coli O55:B5

biological source

Escherichia coli (J5 Rc mutant)

biological source

Escherichia coli (O55:B5)

biological source

Escherichia coli (O55:B5)

biological source

Escherichia coli (O55:B5)

solubility

water: 0.90-1.10 mg/mL, hazy to turbid, colorless to faintly yellow

solubility

H2O: 5 mg/mL, faintly hazy to hazy

solubility

water: 4.90-5.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow

solubility

H2O: 4.90-5.10 mg/mL, faintly hazy to hazy, pale yellow to yellow

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

aqueous solution

form

lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

color

white to faint beige

color

-

color

white to faint yellow

color

yellow to orange

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

Prepared by phenol-chloroform-petroleum ether extraction.
The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

comparable product

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Beschreibung
Preisangaben

pictograms

Skull and crossbones
GHS06

signalword

Danger

hcodes

H300

Hazard Classifications

Acute Tox. 2 Oral

Lagerklasse

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
0000439787
0000439787
0000476051
0000476051
0000406509

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M Nashleanas et al.
Infection and immunity, 68(3), 1428-1434 (2000-02-26)
The ability to activate macrophages in vitro for nitric oxide production and killing of Leishmania major parasites is dependent on tumor necrosis factor, although L. major-infected mice lacking the TNF receptor p55 (TNFRp55(-/-) mice) or both the TNFRp55 and TNFRp75
Xiaoyu Zhang et al.
Oncology letters, 12(2), 1034-1040 (2016-07-23)
Hypoxia-inducible factor-1α (HIF-1α) and toll-like receptor 4 (TLR4) are involved in numerous mechanisms of cancer biology, including cell proliferation and survival; however the interaction of the two factors under hypoxic conditions remains unclear. The present study investigated the in vitro
Sophie E Hussey et al.
Bioscience reports, 33(1), 37-47 (2012-10-12)
Emerging evidence suggests that TLR (Toll-like receptor) 4 and downstream pathways [MAPKs (mitogen-activated protein kinases) and NF-κB (nuclear factor κB)] play an important role in the pathogenesis of insulin resistance. LPS (lipopolysaccharide) and saturated NEFA (non-esterified fatty acids) activate TLR4
Y Wannemuehler et al.
Journal of clinical microbiology, 27(12), 2881-2883 (1989-12-01)
A monoclonal antibody to the lipopolysaccharide of Moraxella bovis is described. In an indirect fluorescent-antibody test, this monoclonal antibody reacted with 39 of 39 strains of M. bovis tested and did not react with 26 nonfermenting gram-negative coccobacilli other than

Artikel

Lipopolysaccharides for Research – Structure, Function & Applications

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Global Trade Item Number

SKUGTIN
L5014-10MG04061833274927
L5014-50MG04061833274934
L5014-5MG04061833957851

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