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L2880

Sigma-Aldrich

Lipopolysaccharide aus Escherichia coli O55:B5

Lipopolysaccharides from Escherichia coli O55:B5

purified by phenol extraction

Synonym(e):

LPS

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10 MG
€ 95,70
25 MG
€ 200,00
100 MG
€ 433,00
500 MG
€ 1.470,00

€ 95,70

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10 MG
€ 95,70
25 MG
€ 200,00
100 MG
€ 433,00
500 MG
€ 1.470,00

About This Item

CAS-Nummer:
93572-42-0
EG-Nummer:
297-473-0
MDL-Nummer:
MFCD00164401
UNSPSC-Code:
12352201
NACRES:
NA.25

€ 95,70

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Biologische Quelle

Escherichia coli (O55:B5)

Qualitätsniveau

300

Form

lyophilized powder

Aufgereinigt durch

phenol extraction

Methode(n)

cell based assay: suitable

Verunreinigungen

<3% Protein (Lowry)

Farbe

off-white to tan

Löslichkeit

water: soluble

Anwendung(en)

cell analysis
clinical research
life science and biopharma

Versandbedingung

ambient

Lagertemp.

2-8°C

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Verwandte Kategorien

Allgemeine Beschreibung

Lipopolysaccharide (LPS) sind Glykolipide,[1] eine Kombination von Kohlenhydrateinheiten, die mit einzelnen oder mehreren Fettsäuren verbunden sind und sich in der Zellwand gramnegativer Bakterien befinden.[2] LPS sind wesentlicher Bestandteil der äußeren Membran, wobei die LPS-Struktur aus Lipid A, einem Phospholipid auf Glucosaminbasis, einem Oligosaccharid mit kurzem Kern und einem O-Antigen, einem distalen Polysaccharid, besteht.[3]

Anwendung

Das Lipopolysaccharid aus Escherichia coli O55:B5 wird wie folgt verwendet:
  • zur Stimulierung von Hepatozyten und nicht-parenchymalen Zellen[4]
  • zur Stimulierung der Milz und Untersuchung der Expression des Malitaf-Gens bei der Schnauzenbrasse[5]
  • im Rahmen des Concanavalin-A- (ConA) und D-Galactosamin/Lipopolysaccharid-Modells (D-GalN/LPS) der fulminanten Hepatitis zur Untersuchung der Wirkung des Naturstoffs Hesperetin[6]
  • zur Überprüfung der Zelllebensfähigkeit von aus menschlichen Chorionzotten gewonnenen mesenchymalen Stammzellen (hCMSCs) mittels MTT-Test (Methylthiazolyltetrazolium – MTT)[7]
  • zur Induzierung von Oligodendrozyten-Toxizität für die Bewertung der neuroprotektiven Wirkungen einiger Verbindungen[8]
  • zur Induzierung von Sarkopenie bei Ratten, um die antioxidative Wirkung von mit Curcumin beladenem hydrophobem oberflächenmodifiziertem Hydroxyapatit zu untersuchen[9]
Lipopolysaccharide (LPS) sind charakteristische Bestandteile der Zellwand von gramnegativen Bakterien. LPS und seine Lipid-A-Gruppe stimulieren Zellen des angeborenen Immunsystems durch den Toll-like-Rezeptor 4 (TLR4). TLR4 gehört zur Toll-like-Rezeptor-Proteinfamilie, die häufige Pathogen-assoziierte molekulare Strukturen (PAMPs) erkennt.

Biochem./physiol. Wirkung

Lipopolysaccharide (LPS) bilden eine schützende Permeabilitätsbarriere, die das Eindringen von Antibiotika und Gallensalzen blockiert, die gramnegative Bakterien von Natur aus resistent machen. LPS ist an der bakteriellen Pathogenität beteiligt, da das Immunsystem des Wirts in erster Linie auf die LPS-Komponente der Bakterien trifft. LPS ist ein Endotoxin und ein hoch immunogenes Antigen, dass das Immunsystem stärken kann. Das Lipid A von LPS stimuliert Wirtszellen über Toll-like-Rezeptoren 4 (TLR4).[1]

Sonstige Hinweise

Gewinner des CiteAb Award 2024 in der Kategorie „Supplier Succeeding in Parkinson′s Research“ (Erfolgreiche Anbieter in der Parkinson-Forschung)
Füllen Sie bitte dieses Formular aus, um weitere Informationen zu unserem Sortiment an Biochemikalien zu erhalten.
Für eine optimale Rekonstitution von LPS-Produkten ist vor Gebrauch unabhängig vom Behältertyp eine intensive Vortexmischung erforderlich. Die Konzentration der Lagerlösung muss mindestens 1 mg/ml betragen. Obwohl (silanisierte) Borosilikat-Behälter empfohlen werden, können auch Kunststoffbehälter aus Polypropylen (PP) verwendet werden.
Für einen umfassenden Überblick über unser vielfältiges Angebot an Lipopolysacchariden für Ihre Forschung empfehlen wir Ihnen den Besuch unserer Seite zur Kategorie Kohlenhydrate.

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Lipopolysaccharide aus Escherichia coli O55:B5, purified by ion-exchange chromatography, TLR ligand tested

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Lipopolysaccharide aus Escherichia coli O55:B5, γ-irradiated, BioXtra, suitable for cell culture

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Lipopolysaccharide aus Escherichia coli O55:B5, purified by gel-filtration chromatography

L2880

Lipopolysaccharide aus Escherichia coli O55:B5, purified by phenol extraction

F8666

Lipopolysaccharide aus Escherichia coli O55:B5, FITC conjugate

L5418

Lipopolysaccharide aus Escherichia coli O55:B5, Ready Made solution, 1 mg/mL

Piktogramme

Skull and crossbones
GHS06

Signalwort

Danger

H-Sätze

H300

Gefahreneinstufungen

Acute Tox. 2 Oral

Lagerklassenschlüssel

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
0000428249
0000428249
0000428255
0000428255
0000428250

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Yun Feng et al.
Stem cell research & therapy, 11(1), 2-2 (2020-01-05)
ALI/ARDS is the major cause of acute respiratory failure in critically ill patients. As human chorionic villi-derived MSCs (hCMSCs) could attenuate ALI in the airway injury model, and liraglutide, glucagon-like peptide 1 (GLP-1) agonist, possesses anti-inflammatory and proliferation promotion functions
Ya-Jyun Liang et al.
Antioxidants (Basel, Switzerland), 10(4) (2021-05-01)
Oxidative stress and later-induced chronic inflammation have been reported to play an important role on the progression of sarcopenia. Current treatments for sarcopenia are mainly administered to patients whom sarcopenia already developed. However, there has been no promising results shown
Outer membrane permeability and antibiotic resistance
Delcour A H, et al.
Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 1794(5) , 808-816 (2009)
A liver microphysiological system of tumor cell dormancy and inflammatory
responsiveness is affected by scaffold properties
Clark AM
Cancer Discovery null
Promising neuroprotective effects of ?-caryophyllene against LPS-induced oligodendrocyte toxicity: A mechanistic study
Askari V R and Shafiee-Nick R
Biochemical Pharmacology, 159, 154-171 (2019)
Alle zugehörigen Dokumente anzeigen

Artikel

Lipopolysaccharides for Research – Structure, Function & Applications

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

1 Review This action will navigate to reviews.

Questions

1–9 of 9 Questions  

  1. I recently purchased this item and I would like to understand how lethal/toxic/dangerous is for humans once diluted to 0.5mg/ml. I would need to inject ip fish with a certain volume from that stock solution.

    1 answer

    1. Toxicological information, when available, is provided in section 11 of the safety data sheet. It is unclear which fish model is being used to study toxicity. Consulting scientific literature is recommended for these details. Below is some information that may be useful to address the question.

      https://www.sigmaaldrich.com/sds/sigma/l2880?userType=anonymous

      https://www.tandfonline.com/doi/abs/10.1080/10934529.2012.695254

      https://www.mdpi.com/1420-3049/19/2/2390

      https://www.sigmaaldrich.com/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharides?srsltid=AfmBOoo1e2mKmwy-vXjZvAmV-Epz80sS4TfN11C2ShOHlc0FPWkb9_2n

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  4. Hi, Can you provide the information regarding molecular weight of this LPS?

    1 answer

    1. The molecular weight of this product has not been determined. In general, the molecular weight of native LPS may range from 1 – 4 million Daltons or higher. When treated with SDS and heat, the molecular weight of LPS may range from 50 to 100 kDa.

      Helpful?

  5. What is the number of acetyl groups attached to the lipid A moiety?

    1 answer

    1. The acyl chain length and number of acyl groups may vary between bacterial species. Certain researchers claim there are 6 acyl groups for LPS from this strain of bacteria. Please refer to those peer reviewed papers below for more information.

      https://www.sciencedirect.com/science/article/pii/S0939641122002338#:~:text=%5B14%5D%2C%20%5B42%5D,in%20a%20single%20LPS%20structure.

      https://pubmed.ncbi.nlm.nih.gov/16934960/

      Helpful?

  6. Can you provide information on the solubility and solution stability of this product?

    1 answer

    1. LPS products in solution are expected to be stable in phosphate buffered saline at a concentration of 1 mg/mL when stored at 4 °C for at least one month. For long-term storage, it is recommended to use frozen aliquots. In order to prevent LPS binding to glass, it is advisable to store the solution in silanized containers. If glass containers are used, it is recommended to vortex the solutions for at least 30 minutes to dissolve any bound LPS.

      Helpful?

  7. Does the bacterial strain have specific information available, such as whether it originates from a private collection or if there is an associated ATCC number?

    1 answer

    1. Unfortunately, the specific strains used to make L2880 are considered proprietary.

      Helpful?

  8. hello, I was wondering what the best way to reconstitute this LPS is

    1 answer

    1. LPS products can be reconstituted in phosphate buffered saline at 1 mg/mL.

      Helpful?

  9. Which LPS grade should be used to induce neurotoxicity in rats?

    1 answer

    1. There is no Lipopolysaccharide product offered specifically for the induction of neurotoxicity in rats. Escherichia coli strain O55:B5 has been used to induce oligodendrocyte toxicity to evaluate the neuroprotective effects of certain compounds. In addition, Escherichia coli strain O111:B4 has been cited in the literature as suitable for LPS-induced neurotoxicity. For use with animal models, LPS purified by gel filtration is most commonly used to eliminate the risk of any residual solvents.

      Please see the links below to review the E. coli strains O111:B4 and O55:B5 that have been purified by gel filtration:
      Lipopolysaccharides – Glycobiology
      https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/research-and-disease-areas/cell-signaling/lipopolysaccharides
      Lipopolysaccharides – selection table
      https://www.sigmaaldrich.com/US/en/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharides

      Helpful?

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