H3917

Heparinase I and III Blend from Flavobacterium heparinum

lyophilized powder, stabilized with ∼ 25% (w/w) bovine serum albumin, ≥200 unit/mg protein (enzyme + BSA)

• Synonym(e): Heparinase I and Heparinase III blend
• UNSPSC Code: 12352204
• NACRES: NA.54
• EG-Nummer: 4.2.2.7 (BRENDA, IUBMB)
• Specific activity: ≥200 units/mg protein
• Biological source: bacterial (Flavobacterium heparinum)
• Concentration: ≥200 unit/mg protein (enzyme + BSA)

Eigenschaften

• biological source: bacterial (Flavobacterium heparinum)
• Quality Segment: 200
• conjugate: conjugate (Glucosaminoglycan)
• form: lyophilized powder
• specific activity: ≥200 units/mg protein
• concentration: ≥200 unit/mg protein (enzyme + BSA)
• shipped in: dry ice
• storage temp.: −20°C

Beschreibung

General description

Heparinase is an inducible, non-extracellular heparin-degrading enzyme. Three types of heparinises are produced by Flavobacterium heparinum and contains specific sequences of heparin.[1]

Application

Heparinase I and III Blend from Flavobacterium heparinum has been used in:

• the digestion of heparan sulfate from ovine vitreous
• human embryonic kidney cells
• glycosaminoglycans from arterial tissues
• P0 retinae digestion

Biochem/physiol Actions

Heparin-degrading lyase that recognizes heparin sulfate proteoglycan as its primary substrate.

Heparinase I and III plays vital role in various biological processes: modulate cell-growth factor interactions, cell-lipoprotein interactions, neovascularization.[2][3][4] It cleaves highly sulphated polysaccharide chains in presence of 2-O-sulfated α-L-idopyranosyluronic acid and β-D-glucopyranosyluronic acid residues of polysaccharides.[5]

Packaging

Sold on the basis of Heparinase I units

Other Notes

Enzyme Commission Numbers: 4.2.2.7 Hep I and 4.2.2.8 Hep III

One unit will form 0.1 micromole of unsaturated uronic acid per hour at 7.5 at 25 degrees C using Heparin, Sodium as substrate for heparinase I.

One unit will form 0.1 micromole of unsaturated uronic acid per hour at 7.5 at 25 degrees C using bovine kidney Heparan, Sulfate as substrate for heparinase III.

One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units. Package sizes are sold in Sigma units.

Sicherheitshinweise

• Lagerklasse: 13 - Non Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable