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D8900

Dulbecco Modifiziertes Eagle-Medium/Nährstoffmischung F-12 Ham

With L-glutamine and 15 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

Synonym(e):

DME/F-12, 1:1 Mischung

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PackungsgrößeSKUVerfügbarkeitPreis
10 x 1 L
Warenkorb auf Verfügbarkeit prüfen
€ 111,00
10 L
Warenkorb auf Verfügbarkeit prüfen
€ 111,00
50 L
Warenkorb auf Verfügbarkeit prüfen
€ 426,00

Über diesen Artikel

UNSPSC Code:
12352207
NACRES:
NA.71
MDL number:

€ 111,00


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Quality Segment

form

powder

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: 0.055 g/L
HEPES: 15 mM
NaHCO3: no
L-glutamine: yes
glucose: 3.15 g/L
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is suitable for the growth of a wide range of cell types in low serum conditions. It has been used:

  • as a component of the culture medium for endometrial explants in vitro culture
  • to immerse chitosan-gelatin-glycerol phosphate hydrogels for cytotoxicity evaluation
  • to culture nucleus pulposus (NP) cells to culture human articular chondrocytes for evaluating the toxicity of baicalein

Preparation Note

Formulated to contain 15.6 grams of powder per liter of medium.
Supplement with 1.2 g/L sodium bicarbonate.

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Dieser Artikel
D0547D2906D8437
form

powder

form

powder

form

powder

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: 0.055 g/L
HEPES: 15 mM
NaHCO3: no
L-glutamine: yes
glucose: 3.15 g/L
phenol red: yes

components

NaHCO3: no
sodium pyruvate: 0.11 g/L
HEPES: no
phenol red: yes
glucose: 3.15 g/L
L-glutamine: yes

components

NaHCO3: no
L-glutamine: yes
phenol red: no
sodium pyruvate: 0.055 g/L
glucose: 3.15 g/L
HEPES: 15 mM

components

glucose: 3.15 g/L
HEPES: yes
L-glutamine: yes
phenol red: yes
sodium pyruvate: 0.055 g/L

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

Quality Level

500

Quality Level

500

Quality Level

500

Quality Level

400


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Lagerklasse

11 - Combustible Solids

wgk

WGK 1



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Protokolle

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Artikel

Nutrient Mixture F-12 Ham Formulation

High-quality ultrapure water that is endotoxin-free is recommended for mammalian cell culture as it support cell viability and prevents contamination.


Sang Hwan Kim et al.
PeerJ, 7, e6344-e6344 (2019-02-08)
Here we investigated the expressions of apoptosis-associated genes known to induce programed cell death through mRNA expressions of two matrix metalloproteinases (MMPs) that are involved in the degradation of collagen and basal membrane in luteal cells cultured in the treatment
Elena Ruggeri et al.
Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 23(6), 1197-1206 (2017-12-07)
Confocal microscopy was used to image stages of equine zygote development, at timed intervals, after intracytoplasmic sperm injection (ICSI) of oocytes that were matured in vivo or in vitro. After fixation for 4, 6, 8, 12, or 16 h after
Misa Nakao et al.
Ultrasound in medicine & biology, 45(5), 1306-1315 (2019-02-26)
Cellular aggregates that mimic cell-cell interactions in vitro are essential for biological research. This study introduces a method to form large scaffold-free 3-D aggregates in a clinically ubiquitous cell culture dish using kilohertz-order ultrasound standing wave trapping (USWT). We fabricated



Global Trade Item Number

SKUGTIN
D8900-50L04061835568376
D8900-10X1L04061833590089
D8900-50K04061832954301
D8900-10L04061833590072

Questions

  1. What is the molarity of glucose when reconstituted with water?

    1 answer
    1. Upon reconstitution, the molarity of glucose is 17.5 mM (3.15 g/L).

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