D2906

DMEM/F-12

With ʟ-glutamine, sodium pyruvate and HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture

• Synonym(e): DME/F-12, 1:1 Mischung
• UNSPSC Code: 12352207
• NACRES: NA.75
• MDL number: MFCD00217417

Eigenschaften

• Produktname: Dulbecco Modifiziertes Eagle-Medium/Nährstoffmischung F-12 Ham, With L-glutamine and 15 mM HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture
• Quality Segment: 500
• form: powder
• technique(s): cell culture | mammalian: suitable
• components: NaHCO₃: no; L-glutamine: yes; phenol red: no; sodium pyruvate: 0.055 g/L; glucose: 3.15 g/L; HEPES: 15 mM
• shipped in: ambient
• storage temp.: 2-8°C

Beschreibung

General description

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is suitable for the growth of a wide range of cell types in low serum conditions. The removal of phenol red is often preferred by investigators working with clonal and stem cell cultures.

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used to culture:

• Hep-2 cells
• mouse fibroblast L‐cells
• L‐ Wnt family member 3A (Wnt3a) cells
• primary buffalo embryonic fibroblast (BEF)

Preparation Note

Formulated to contain 15.6 grams of powder per liter of medium.

Supplement with 1.2 g/L sodium bicarbonate.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1