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B2025

Brilliant Blue G - Colloidal Concentrate

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€ 92,10

Über diesen Artikel

NACRES:
NA.85
UNSPSC Code:
12352200

€ 92,10


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form

solution

Quality Segment

storage temp.

room temp

General description

Coomassie Brilliant Blue stain is widely used for the detection of proteins in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). Staining with brilliant blue G is called as blue silver staining due to its improved sensitivity close to silver staining method. Coomassie Brilliant Blue G stain associates with proteins via hydrophobic interaction. It is effective in staining anterior capsule in cornea with relatively less toxic effects.

Application

Brilliant Blue G - Colloidal Concentrate has been used in the staining
  • protein fragments post in vitro pepsin digestion
  • ovarian tumor fluid proteome prior to profiling
  • the transcription factor (TFIID) complex from Saccharomyces cerevisiae

Preparation Note

The suspension will contain 0.1% (w/v) Brilliant Blue G, 0.29 M phosphoric acid and 16% saturated ammonium sulfate after diluting to 1 liter with 800 mL water.

Analysis Note

This product has been tested for suitability on SDS-PAGE.

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Dieser Artikel
B8522B07701.15444
form

solution

form

liquid

form

powder

form

-

Quality Level

200

Quality Level

-

Quality Level

200

Quality Level

100

storage temp.

room temp

storage temp.

2-8°C

storage temp.

room temp

storage temp.

no temp limit


pictograms

Corrosion

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Lagerklasse

12 - Non Combustible Liquids


Zulassungslistungen

Zulassungslistungen werden hauptsächlich für chemische Produkte erstellt. Für nicht-chemische Produkte können hier nur begrenzte Angaben gemacht werden. Kein Eintrag bedeutet, dass keine der Komponenten gelistet ist. Es liegt in der Verantwortung des Benutzers, die sichere und legale Verwendung des Produkts zu gewährleisten.

7783-20-2

CAS No.


Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number

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Questions

1–6 of 6 Questions  
  1. What is the solution stability of Product B2025, Brilliant Blue G?

    1 answer
    1. Once diluted the solution should be stable indefinitely.  It should be stored in the refrigerator and shaken well before each use.  The solution tends to precipitate on standing and heat accelerates precipitation.  Therefore, do not leave the bottle at room temperature overnight.

      Helpful?

  2. How would you recommend that I reconstitute Product B2025, Brilliant Blue G?

    1 answer
    1. One bottle of the product makes 1 liter of working solution.  The suspension will contain 0.1% (w/v) Brilliant Blue G, 0.29 M phosphoric acid and 16% saturated ammonium sulfate after diluting to 1 Liter with 800 mL water.

      Helpful?

  3. How is Product B2025, Brilliant Blue G, better than other Coomassie Blue dyes?

    1 answer
    1. The major advantage of the colloidal form is that it gives less background staining that the "regular" non-colloidal Coomassie Brilliant Blue G and Brilliant Blue R stains. Sensitivity can be markedly increased by double staining, first with Coomassie Brilliant Blue G Colloidal, then Rapid Silver Stain.

      Helpful?

  4. Can you use Product B2025, Brilliant Blue G, with nitrocellulose?

    1 answer
    1. Use with nitrocellulose is not recommended, due to the methanol.

      Helpful?

  5. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  6. What is the detection limit of Product B2025, Brilliant Blue G?

    1 answer
    1. The detection limit for proteins is about 50 ng of protein. However, proteins pick up stains differently so this is a general description and may not apply to all proteins

      Helpful?

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