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Merck

85881

Supelco

Streptavidin, immobilisiert auf Agarose CL-4B

suitable for affinity chromatography

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About This Item

UNSPSC-Code:
12190000
NACRES:
NA.21

Form

suspension

Qualitätsniveau

Enthält

0.02% NaN3 as preservative

Methode(n)

affinity chromatography: suitable

Kapazität

≥15 μg/mL, packed gel capacity (biotin)

Lagertemp.

2-8°C

Allgemeine Beschreibung

Streptavidin ist über einen C6-Spacer an 4% quervernetzte Agarose-Perlengebunden (1.2 mg Streptavidin/ml gepacktes Gel)

Physikalische Form

50% Suspension in 0.01 M Natriumphosphat-Puffer, pH 7.2, mit 0.15 M Natriumchlorid und 0.02% NaN3

Sonstige Hinweise

Isolation of cell surface glycoprotein by biotinylated concanavalin A and immobilized streptavidin

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves


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Molecular and cellular probes, 46, 101415-101415 (2019-06-23)
Vascular endothelial growth factor (VEGF) can promote cell division, proliferation and migration. In this study, we aimed to investigate roles of ultrasound-mediated destruction of VEGF-targeted and paclitaxel (PTX)-loaded lipid microbubbles (VTPLLM + US) in human breast cancer cells. The activity of MCF-7 cells
J W Buckie et al.
Analytical biochemistry, 156(2), 463-472 (1986-08-01)
An indirect affinity chromatography procedure utilizing biotinylated lectins and designed for the specific isolation of surface glycoproteins is described. The method is illustrated with intact acute leukemic lymphoblastic cells (ALL cells) with biotin-epsilon-aminocaproyl-concanavalin A (biocap-Con A) and streptavidin-Sepharose 4B. Biocap-Con
Romina B Cejas et al.
Biological chemistry, 401(9), 1041-1051 (2020-04-17)
Glycosylation is a very frequent post-translational modification in proteins, and the initiation of O-N-acetylgalactosamine (O-GalNAc) glycosylation has been recently described on relevant nuclear proteins. Here we evaluated the nuclear incorporation of a second sugar residue in the biosynthesis pathway of
Yen-Jung Chen et al.
Antioxidants & redox signaling, 31(2), 109-126 (2018-11-13)
Aims: The coordination of neurons to execute brain functions requires plenty of oxygen. Thus, it is not surprising that

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