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07-520

Anti-eNOS/NOS III Antibody, CT

Upstate®, from rabbit

Synonym(e):

Constitutive NOS, Endothelial NOS, Endothelial NOS, Endothelial NOS, endothelial nitric oxide synthase, nitric oxide synthase 3 (endothelial cell)

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PackungsgrößeSKUVerfügbarkeitPreis
200 μg
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€ 569,00

Über diesen Artikel

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
WB
Citations:
15

€ 569,00


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biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, pig, guinea pig, rat, canine, human, bovine

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

bovine ... Nos3(287024)
dog ... Nos3(403784)
guinea pig ... Nos3(100135577)
human ... NOS3(4846)
mouse ... Nos3(18127)
pig ... Nos3(397557)
rat ... Nos3(24600)

General description

135 kDa
Endothelial NOS (eNOS), also known as Nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is a nitric oxide synthase. It generates NO in blood vessels and is involved with regulating vascular function. A constitutive Ca2+ dependent NOS provides a basal release of NO. eNOS is associated with plasma membranes surrounding cells and the membranes of Golgi bodies within cells.

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 1181-1194 (C-QERQLRGAVPWAFD) of human eNOS/NOS III. The immunizing sequence has 10/11 identical amino acids in mouse, guinea pig, bovine, pig, rat, and dog.

Application

Anti-eNOS/NOS III Antibody, CT detects level of eNOS/NOS III & has been published & validated for use in WB.

Biochem/physiol Actions

Predicted to cross-react with mouse, guinea pig, pig, rat and dog based on sequence homology.
Recognizes eNOS/NOS III, Mr ~135 kDa.

Physical form

Format: Purified
Purified rabbit polyclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide before the addition of glycerol to 30%. Liquid at -20°C.

Analysis Note

Control
Platelets, placenta, liver and kidney.
Routinely evaluated by western blot in RIPA lysates from HUVEC cells.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected eNOS/NOS III in RIPA lysates from HUVEC cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Dieser Artikel
07-428-I07-571-I04-811
conjugate

unconjugated

conjugate

-

conjugate

unconjugated

conjugate

-

species reactivity

mouse, pig, guinea pig, rat, canine, human, bovine

species reactivity

human

species reactivity

mouse, rat

species reactivity

bovine

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

culture supernatant

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

monoclonal

Gene Information

bovine ... Nos3(287024)
dog ... Nos3(403784)
guinea pig ... Nos3(100135577)
human ... NOS3(4846)
mouse ... Nos3(18127)
pig ... Nos3(397557)
rat ... Nos3(24600)

Gene Information

human ... NOS3(4846)

Gene Information

mouse ... Nos1(18125)
rat ... Nos1(24598)

Gene Information

mouse ... Nos3(287024)


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Lagerklasse

10 - Combustible liquids

wgk

WGK 1



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Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Verwandter Inhalt

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Apigenin inhibits the TNF?-induced expression of eNOS and MMP-9 via modulating Akt signalling through oestrogen receptor engagement.
Daniela Palmieri,Patrizia Perego,Domenico Palombo
Molecular and Cellular Biochemistry null
Suzanne D Burke et al.
The Journal of clinical investigation, 126(7), 2561-2574 (2016-06-09)
Preeclampsia is a hypertensive disorder of pregnancy in which patients develop profound sensitivity to vasopressors, such as angiotensin II, and is associated with substantial morbidity for the mother and fetus. Enhanced vasoconstrictor sensitivity and elevations in soluble fms-like tyrosine kinase
Harita Dharaneeswaran et al.
Circulation research, 115(2), 238-251 (2014-05-31)
Forkhead box-O transcription factors (FoxOs) transduce a wide range of extracellular signals, resulting in changes in cell survival, cell cycle progression, and several cell type-specific responses. FoxO1 is expressed in many cell types, including endothelial cells (ECs). Previous studies have



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