Safranin O stain for cartilage requires a specific procedure involving fixative, deparaffinization, staining, and mounting. The use of acetic acid after staining sets the stain to the tissue.
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S8884
Safranin O
Dye content, ≥80%, certified by the Biological Stain Commission, powder
Synonym(e):
Basic Red 2, Gossypimin, Safranin T, Safranin Y oder A, Woll-Rot
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 25 g | Warenkorb auf Verfügbarkeit prüfen | € 119,00 | |
| 100 g | Warenkorb auf Verfügbarkeit prüfen | € 412,00 |
Über diesen Artikel
Empirische Formel (Hill-System):
C20H19ClN4
CAS-Nummer:
Molekulargewicht:
350.84
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
207-518-8
MDL number:
Colour Index Number:
50240
Beilstein/REAXYS Number:
3924099
Technischer Dienst
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Unterstützung erhaltenProduktname
Safranin O, certified by the BSC
agency
certified by the BSC
Quality Segment
form
powder
composition
Dye content, ≥80%
technique(s)
microbe id | staining: suitable
color
green to very dark green
solubility
H2O: 1 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N
InChI
1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H
InChI key
OARRHUQTFTUEOS-UHFFFAOYSA-N
General description
Safranin O is a metachromatic, cationic dye. It is used as a counterstain in Gram staining. The stain colors Gram-negative bacteria pink to red and has no effect on Gram-positive bacteria. It is also used to assess glycosaminoglycans (GAGs) in cartilage. The dye imparts an intense red to GAGs. It easily binds to polyanions.
Application
Safranin O has been used in the staining of extraocular muscle mesenchymal stem cells (EOM-MSC) for the detection of cartilage, mucin and mast cell granules It has also been used as a mediator dye in cytochrome P450 spectral assay.
Zertifiziert für die Flemming'sche Dreifachfärbung von Chromosomen, Färbung gramnegativer Bakterien bei der Gramfärbung, Safranin-Echtgrün FCF-Färbung von Pflanzenzellen.
Safranin O has been used to stain matrix proteoglycans to study cartilaginous tissue structural integrity in mice. It has also been used to stain chondrogenically differentiated human bone marrow stromal cells.
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Dieser Artikel | |||
|---|---|---|---|
| technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable | technique(s) titration: suitable | technique(s) cell based assay: suitable, cell culture | stem cell: suitable, cell differentiation: suitable |
| color green to very dark green | color - | color - | color - |
| form powder | form powder | form powder or crystals | form - |
| solubility H2O: 1 mg/mL | solubility water: 1 mg/mL, clear, dark red to very dark red and red purple | solubility methanol: 0.01 g/10 mL, red | solubility - |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. - |
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signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Verwandter Inhalt
Novel markers of osteogenic and adipogenic differentiation of human bone marrow stromal cells identified using a quantitative proteomics approach
CeciliaGraneli
Stem Cell Research (2014)
Perturbations in the HDL metabolic pathway predispose to the development of osteoarthritis in mice following long-term exposure to western-type diet
I.-E.Triantaphyllidou
Osteoarthritis and Cartilage / OARS, Osteoarthritis Research Society (2013)
Maria Dannessa Delost
Introduction to Diagnostic Microbiology for the Laboratory Sciences (2014)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| S8884-100G | 04061835574049 |
| S8884-25G | 04061836961138 |
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Is there a staining protocol available for staining cartilage, particularly for decalcified bone (joint tissue) using CalX, and subsequent staining with Fast green and Safranin O?
1 answer-
Helpful?
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