S0149

Streptolysin O aus Streptococcus pyogenes

From Streptococcus pyogenes, Thiol-activated toxin, lyophilized powder

• CAS-Nummer: 98072-47-0
• UNSPSC Code: 12352200
• NACRES: NA.77
• EC Number: 308-500-3
• MDL number: MFCD00132389
• Form: lyophilized powder
• Quality level: 200

Eigenschaften

• Produktname: Streptolysin O aus Streptococcus pyogenes, γ-irradiated
• biological source: Streptococcus pyogenes
• Quality Level: 200
• sterility: γ-irradiated
• form: lyophilized powder
• application(s): cell analysis
• storage temp.: 2-8°C
• Gene Information: Streptococcus pyogenes ... slo(900490)

Beschreibung

Application

Permeabilisiert Membranen, um die Aufnahme großer oder geladener Moleküle in die Zelle zu ermöglichen.

Streptolysin O from Streptococcus pyogenes has been used in streptolysin O (SLO) permeabilization. It has also been used to evaluate the barrier integrity and cytotoxicity of caco-2 and T84 IEC polarized monolayers.

Biochem/physiol Actions

Thiol-aktiviertes Toxin, dass die Zellmembranen von Tieren permeabilisiert.

Thiol-aktiviertes Toxin, dass die Zellmembranen von Tieren permeabilisiert. Das Protein bindet als Monomer an das Cholesterin von Membranen und polymerisiert in der Folge zu großen, bogen- und ringförmigen Strukturen, die Poren > 12 nm umgeben.

Streptolysin O (SLO), obtained from Streptococcus pyogenes has the capability to block osteoclastogenesis, induced by receptor activator of nuclear factor κ-Β ligand (RANK) through the NF-κB signaling pathway. Streptococcus pyogenes (GAS) is a bacterial strain that results in bacterial arthritis, osteomyelitis and implant infections.

Preparation Note

Prepared from S 5265

Other Notes

One unit will cause 50% lysis of 2% red blood cell suspensions in phosphate buffered saline, pH 7.4, after incubation at 37 °C for 30 min.

Sicherheitshinweise

• pictograms: GHS06
• signalword: Danger
• hcodes: H300 + H310 + H330
• pcodes: P262 - P280 - P301 + P310 + P330 - P302 + P352 + P310 - P304 + P340 + P310
• Hazard Classifications: Acute Tox. 1 Dermal - Acute Tox. 1 Inhalation - Acute Tox. 1 Oral
• Lagerklasse: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)