Upon verification, the laboratory has not characterized the biological activity of SAE0089. However, it is known to be reversibly inactivated by oxidation, where the active form is present in a reduced format. Therefore, just prior to application, one could activate it by incubation with DTT for about 15 minutes at 37 degrees Celsius.
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SAE0089
Streptolysin O aus Streptococcus pyogenes
≥1,000,000 units/mg protein, recombinant, lyophilized powder, expressed in E. coli
Synonym(e):
Streptolysin O from Streptococcus pyogenes, SLO
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
100 kU | Warenkorb auf Verfügbarkeit prüfen | € 204,00 |
Über diesen Artikel
Assay:
≥95% (SDS-PAGE)
Technischer Dienst
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Unterstützung erhaltenassay
≥95% (SDS-PAGE)
specific activity
≥1,000,000 units/mg protein
mol wt
60 kDa
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
General description
Streptolysin O (SLO) is an immunogenic, oxygen-labile toxin, hemolytic exotoxin which is reversibly activated by dithiothreitol. [1] It is released into the extracellular medium along with other toxins, including streptolysin S, during the growth of most strains of group A and many strains of groups C and G Streptococci. [1,2] SLO and Streptolysin S differ from each other in that SLO is immunogenic and oxygen-labile while Streptolysin S is oxygen-stable, nonimmunogenic and only active when associated with a carrier protein.[3] The hemolytic activity of SLO is mediated by formation of multimeric nanopores in cholesterol containing lipid membranes.
SLO may be used for cell permeabilization or hemolysis. The susceptibility of hemolysis by SLO varies significantly for erythrocytes from different animal species.[1] Permeabilization of cells using SLO has been performed on multiple cell types and for various applications. For instance it has been used to introduce antisense oligonucleotides into cultured eukaryotic cells;[3] to investigate the effect of guanine nucleotide analogues on phosphatidylinositol metabolism and protein kinase C (PKC) activation in live human T lymphocytes;[4] to monitor cholesterol oxidation within a membrane lipid bilayer; [5] and to label proteins inside living cells using external fluorophores.[6]
SLO may be used for cell permeabilization or hemolysis. The susceptibility of hemolysis by SLO varies significantly for erythrocytes from different animal species.[1] Permeabilization of cells using SLO has been performed on multiple cell types and for various applications. For instance it has been used to introduce antisense oligonucleotides into cultured eukaryotic cells;[3] to investigate the effect of guanine nucleotide analogues on phosphatidylinositol metabolism and protein kinase C (PKC) activation in live human T lymphocytes;[4] to monitor cholesterol oxidation within a membrane lipid bilayer; [5] and to label proteins inside living cells using external fluorophores.[6]
Application
Permeabilisiert Membranen, um die Aufnahme großer oder geladener Moleküle in die Zelle zu ermöglichen.
Biochem/physiol Actions
Thiol-aktiviertes Toxin, dass die Zellmembranen von Tieren permeabilisiert.
Thiol-aktiviertes Toxin, dass die Zellmembranen von Tieren permeabilisiert. Das Protein bindet als Monomer an das Cholesterin von Membranen und polymerisiert in der Folge zu großen, bogen- und ringförmigen Strukturen, die Poren > 12 nm umgeben.
This product is produced by recombinant expression in Escherichia coli and contains the complete native protein sequence of SLO (Uniport ID: P0DF96 aa 34-571) without any added purification tags and has calculated molecular weight of 60,144 Dalton. The material is lyophilized from a solution containing 20 mM Sodium Hepes pH 7.5, 150 mM Sodium Chloride and 2 mM EDTA.
Other Notes
Lyophilized powder containing Hepes buffer salts and EDTA.
One unit will cause 50% lysis of 50 ul of a 2% human red blood cell suspension in phosphate buffered saline, pH 7.4, at 37 °C for 30 minutes.
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Dieser Artikel | |||
|---|---|---|---|
| assay ≥95% (SDS-PAGE) | assay - | assay - | assay - |
| mol wt 60 kDa | mol wt native 69 kDa | mol wt - | mol wt 180 kDa |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in ambient | shipped in - | shipped in - | shipped in wet ice |
| specific activity ≥1,000,000 units/mg protein | specific activity 25,000-50,000 U/vial | specific activity - | specific activity - |
| UniProt accession no. | UniProt accession no. - | UniProt accession no. - | UniProt accession no. - |
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signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 2 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral
Lagerklasse
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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I purchased SAE0089 (streptolysin o). I see in many protocols and one of your datasheets that Streptolysin o should be treated with 10 mM dithiothreitol before using it. However, I don't see that indication anywhere for SAE0089. Do I still need to treat it with DTT?
1 answer-
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