The long term stability of this material in solution when stored at -20°C has not been determined. Stock solutions stored at -70°C are expected to be stable for one month.
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D0564
Diphtheria Toxin
from Corynebacterium diphtheriae, ≥98% (SDS-PAGE), lyophilized powder, protein synthesis inhibitor
Synonym(e):
DTx
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 mg | Warenkorb auf Verfügbarkeit prüfen | € 1.010,00 |
Über diesen Artikel
Form:
lyophilized powder
Technischer Dienst
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Unterstützung erhaltenProduktname
Diphterietoxin aus Corynebacterium diphtheriae, lyophilized powder, Product is in unnicked form
Quality Segment
form
lyophilized powder
storage temp.
2-8°C
General description
Diphtherietoxin kann als Toxinelement bei der Konstruktion eines Immuntoxins für die zellspezifische Zytotoxizität verwendet werden. Die Translokationsdomäne der bakteriellen Toxine mit natürlichem Endosomen-Fluchtmechanismus wird für die Entwicklung effizienter nichtviraler Vektoren für Anwendungen in der Gentherapie verwendet.
Application
Diphtherietoxin aus Corynebacterium diphtheriae wird wie folgt verwendet:
- zur Depletion von Mikroglia, um posttraumatische Belastungsstörung bei Mäusen zu untersuchen[1]
- als intraperitoneale Injektion in transgenen Mäusen, um die Hybride nach der Fusion von transplantierten hämatopoetischen Stamm- und Vorläuferzellen (HSPC) bei der Ablation zu selektieren[2]
- zur Untersuchung seiner Wirkung auf die auf die Eosinophillinie festgelegten Vorläuferzellen in einem eosinophildefizienten Mäusestamm (iPHIL)[3]
Biochem/physiol Actions
Diphtherietoxin ist ein bakterielles Toxin, das aus Corynebacterium diphtheriae erzeugt wird. Es enthält drei Domänen, die intrazelluläre Wirkungen aufweisen. Die Domänen sind an der Vergiftung der Zelle, der Zelloberflächenbindung und Internalisierung in Endosomen, der Translokation durch die Endosommembran in das Zytosol und die Hemmung der zellulären Proteinsynthese beteiligt. Diphtherietoxin ist nützlich zum Entwickeln von biotechnologischen Werkzeugen und Therapeutika. Es hemmt die Proteinsynthese, indem es die Ribosylierung des Zielproteins durch Adenosindiphosphat (ADP) in Eukaryoten katalysiert.
Physical form
Lyophilisiertes Pulver, das Tris und EDTA enthält.
Preparation Note
Jede mit 0,5 ml sterilem destilliertem Wasser rekonstituierte Flasche enthält ~1 mg Diphtherietoxin in 0,01 M Tris und 0,001 M Na2EDTA, pH 7,5.
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| form lyophilized powder | form solid | form lyophilized powder | form lyophilized powder |
| Quality Level 200 | Quality Level 100 | Quality Level 300 | Quality Level 200 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 1 Inhalation - Acute Tox. 1 Oral
Lagerklasse
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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How long can diphtheria toxin be stored at -30°C after reconstitution?
1 answer-
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How long can the product be stored for at 4C in its original packaging? Before reconstitution? Furthermore, what should it be reconstituted with for best efficiency and long-term effectiveness?
1 answer-
This product is intended for laboratory or research use only, and no formal solution stability studies have been conducted. Before reconstitution, storage at 4°C in its original packaging is recommended, but specific duration guidelines are not provided. For reconstitution, use sterile distilled water to achieve a concentration of 1 mg of diphtheria toxin in 10 mM sodium phosphate with 5% lactose, pH 7.4. After reconstitution, unused toxin can be quick frozen in aliquots on dry ice and stored at -70°C. Thawing should be done quickly at room temperature. Repeated freezing and thawing or maintaining the preparation at 4°C for extended periods are not recommended, as these may lead to increased nicking. For assays using very low concentrations of the toxin, a carrier protein such as human serum albumin is suggested. The preparation migrates as a single major band under non-denaturing conditions, with a slower migrating band comprising less than 5% of the preparation, indicating an aggregate of diphtheria toxin. In the presence of a reducing agent, on SDS gels, the preparation shows a major band of approximately 63,000 daltons corresponding to the intact toxin, and two faster bands of approximately 39,000 and 24,000 daltons corresponding to the B and A fragments, respectively. The degree of nicking is estimated to be less than 5%. It is not recommended to store the solution frozen, and use within 1 month of resuspension is advised. The vial contains salts for TRIS and EDTA at pH 7.5.
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How long is the lyophilized DT stable for if kept in its original packaging at 4C?
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Products that are robust in nature and extremely stable, in the original unopened container, are not assigned an expiration date or retest schedule. These products will have no dating reported on either the label or the Certificate of Analysis. This product falls under that category. The specification sheet is available at this link: https://www.sigmaaldrich.com/specification-sheets/872/957/D0564-BULK________SIGMA____.pdf
Products that are not assigned an expiration or retest date are covered by a warranty period of 1 year from the date of product shipment.
Full Product Dating Information may be reviewed at the link below: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/190/408/product-dating-information-ms.pdf
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What is recommend for reconstituting the product for injection in mice via ip injection? The product instructions advise using sterile distilled water, but the product also contains Tris and EDTA. Most references and research articles recommend using PBS for reconstitution. I am concerned that water might be too hypotonic for the mice and may cause their cells to rupture, but I'm not sure whether the Tris and EDTA can prevent that.
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No test has been conducted on this product for intraperitoneal injection. However, for intraperitoneal injection in mice, normal saline solution appears to be effective as a delivery solvent
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What is the solution stability of Product D0564, Diphtheria Toxin from Corynebacterium diphtheriae?
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Once the product is reconstituted in water, the solution may be stored in aliquots at -20°C. Avoid freeze-thawing. Long-term storage in the refrigerator at 2-8°C is not recommended.
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How stable is Product D0564, Diphtheria Toxin from Corynebacterium diphtheriae, at low concentration?
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For assays employing low concentration of the toxin, the use of a carrier protein like serum albumin is recommended.
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What is the solubility of Product D0564, Diphtheria Toxin from Corynebacterium diphtheriae?
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This product is soluble in water. Reconstitute with the volume of water described under Description on our website page for D0564.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the molecular weight of Product D0564, Diphtheria Toxin from Corynebacterium diphtheriae?
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In the presence of a reducing agent, on SDS gels, this preparation migrates as a major band of approx. mw 63,000 Daltons (corresponding to thte intact toxin) and two faster more lightly stained bands of approx. MW 39,000 and 24,000 Daltons (corresponding to the B and A fragments, respectively). Based on this method, the extent of the degree of nicking in this preparation is estimated to be less than 5%.
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How is Product D0564-1MG, Diphtheria Toxin, packaged?
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Product D0564-1MG, Diphtheria Toxin, is packaged in a small serum vial with a rubber or silicone septum and aluminum crimp top. The product can be reconstituted in the vial by piercing the septum with a syringe fitted with a stainless steel needle.
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