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P8361
Phosphatase, alkalisch Rind
recombinant, expressed in Pichia pastoris, ≥4000 units/mg protein
Synonym(e):
Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 5000 units | Warenkorb auf Verfügbarkeit prüfen | € 518,00 |
Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-631-4
MDL number:
eCl@ss:
42010105
Specific activity:
≥4000 units/mg protein
Recombinant:
expressed in Pichia pastoris
€ 518,00
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
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Unterstützung erhaltenrecombinant
expressed in Pichia pastoris
Quality Level
form
solid
specific activity
≥4000 units/mg protein
mol wt
apparent mol wt 160 kDa
storage temp.
2-8°C
Application
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It is routinely used to dephosphorylate proteins and nucleic acids. It may be used for protein labeling when high sensitivity is required. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase. Product P8361 has been used to dephosphorylate Dishevelled (Dvl) , which is a key component in the Wnt/β-catenin signaling pathway[1].
An assay, using this ALP enzyme, was developed to assess the cryoprotective activity of two proteins CRP-1 and CRP-2. The enzyme has been used for inhibitory studies of cyclic nucleotide analogs that inhibit prostatic acid phosphatase using a fluorogenic assay.[2] It is commonly used as a "reporter" in detection systems, in which it is conjugated to a protein (antibody, streptavidin, etc.) that specifically recognizes a target molecule. Alkaline phosphatase has also been used to dephosphorylate casein.[3][4]
The product is manufactured without the use of any animal source materials during fermentation or purification.
Biochem/physiol Actions
Alkaline phosphatase dephosphorylates proteins and nucleic acids.
Alkaline phosphatase is a dimer consisting of two equal subunits.[5] The enzyme is a glycoprotein containing approximately 12% carbohydrate (6% hexoses and 6% other neutral sugars). Each molecule of alkaline phosphatase contains four zinc atoms and four disulfide bridges.[6] Maximal activity with alkaline phosphatase is achieved in the presence of magnesium.[7] It catalyzes the hydrolysis of phosphate monoesters such as p-nitrophenyl phosphate, phenyl phosphate, phenolphthalein phosphate, α-glycerol phosphate, β-glycerol phosphate, 2-phosphorylglycerate, triosephosphate, glucose-6-phosphate, glucose 1-phosphate, fructose 1-phosphate, fructose 6-phosphate, adenosine 5-phosphate adenosine 3-phosphate, phosphoenolpyruvate, and β-nicotinamide adenine dinucleotide phosphate.[8][9][10] Arsenate, cysteine, iodine, inorganic phosphate, pyrophosphate, diisopropyl phosphate, triphenylphosphate, diisopropyl fluorophosphate, and L-phenylalanine are some of the strong inhibitors of alkaline phosphatase.[8][10][11] The enzyme is most stable in the pH range 7.5-9.5.2. The pH optimum for enzymatic activity is pH 8-10.[6]
Other Notes
One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C.
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Dieser Artikel | |||
|---|---|---|---|
| specific activity ≥4000 units/mg protein | specific activity ≥5,700 DEA units/mg protein | specific activity ~2500 U/mg protein (~10 mg/ml) | specific activity ≥5,500 DEA units/mg protein |
| form solid | form (40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6) | form solution (clear, colorless) | form (Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6) |
| recombinant expressed in Pichia pastoris | recombinant - | recombinant - | recombinant - |
| mol wt apparent mol wt 160 kDa | mol wt dimer ~160 kDa | mol wt - | mol wt dimer ~160 kDa |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level - | Quality Level 200 |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Lagerklasse
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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The activation of alkaline phosphatase by effector molecules. A combined kinetic and hydrodynamic study.
H Neumann et al.
European journal of biochemistry, 109(2), 475-480 (1980-08-01)
Ondrej Bernatik et al.
The Journal of biological chemistry, 286(12), 10396-10410 (2011-02-03)
Dishevelled (Dvl) is a key component in the Wnt/β-catenin signaling pathway. Dvl can multimerize to form dynamic protein aggregates, which are required for the activation of downstream signaling. Upon pathway activation by Wnts, Dvl becomes phosphorylated to yield phosphorylated and
The enzymic dephosphorylation of casein fractions in the presence of 0-18 labelled water.
E B KALAN et al.
Archives of biochemistry and biophysics, 85, 273-275 (1959-11-01)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| P8361-5000UN | 04061834401339 |