79390

Phosphatase, alkalisch aus Kälberdarmschleimhaut

suitable for enzyme immunoassay, solution (clear, colorless), ~2500 U/mg protein (~10 mg/ml)

• Synonym(e): Alkaline Phosphatase aus Kälberdarmschleimhaut
• CAS-Nummer: 9001-78-9
• UNSPSC Code: 12352204
• eCl@ss: 42010105
• EC Number: 232-631-4
• NACRES: NA.54
• MDL number: MFCD00131849
• EG-Nummer: 3.1.3.1 (BRENDA, IUBMB)
• Specific activity: ~2500 U/mg protein (~10 mg/ml)
• Biological source: bovine (calf) intestinal mucosa

Eigenschaften

• biological source: bovine (calf) intestinal mucosa
• form: solution (clear, colorless)
• specific activity: ~2500 U/mg protein (~10 mg/ml)
• technique(s): enzyme immunoassay: suitable
• impurities: 0.1 mM ZnCl₂, 1 mM MgCl₂, 3 M NaCl, 30 mM triethanolamine
• pH: ~7.6
• storage temp.: 2-8°C

Beschreibung

Application

Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.

Biochem/physiol Actions

Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.

Preparation Note

Die Lösung kann ohne Dialyse für Enzymimmunassay eingesetzt werden.

Other Notes

1 U entspricht der Enzymmenge, die 1 μmol 4-Nitrophenyl-phosphat pro Minute bei pH 9.8 und 37°C hydrolysiert

Kann Verkaufsbeschränkungen unterliegen.

Used in highly sensitive (pg/m) enzyme immunoassays with LCEC detection of phenol; Comparison of three methods for conjugating alkaline phosphatase to human placental lactogen[1]; Preparation of enzyme-antibody conjugates[2]; Liposome immunoassay with antigen-coupled liposomes containing alkaline phosphatase[3]

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)