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M1882

Myoglobin aus Pferdeherz

≥90% (SDS-PAGE), essentially salt-free, lyophilized powder

Myoglobin from equine heart
1 of 1 reviewers received a sample product or took part in a promotion

Synonym(e):

Myoglobin from horse heart

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Ihnen/SKUVerfügbarkeitPreis
250 mg
Warenkorb auf Verfügbarkeit prüfen
€ 111,00
1 g
Warenkorb auf Verfügbarkeit prüfen
€ 356,00
5 g
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€ 1.420,00
10 g
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€ 2.470,00

Über diesen Artikel

CAS-Nummer:
100684-32-0
UNSPSC Code:
12352202
EC Number:
309-705-0
NACRES:
NA.61
MDL number:
MFCD00131643
Form:
essentially salt-free, lyophilized powder
Assay:
≥90% (SDS-PAGE)
Biological source:
equine heart

€ 111,00

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Unterstützung erhalten

biological source

equine heart

Quality Segment

200

assay

≥90% (SDS-PAGE)

form

essentially salt-free, lyophilized powder

Iron content

≥0.20%

technique(s)

MALDI-MS: suitable

UniProt accession no.

P68082

storage temp.

−20°C

Gene Information

horse ... MB(100054434)

Application

Myoglobin aus dem Herzmuskel des Pferdes ist geeignet für folgende Anwendungen:
  • Spektralmessungen mit Beckman DU-50- oder Gilford 2400-Spektrophotometern[1]
  • Analyse der Sekundärstruktur von Proteinen in H2O-Lösung unter Verwendung von Einzel-ATR-FT-IR-Mikroskopie (Fourier-Transformations-Infrarot-Spektroskopie mit abgeschwächter Totalreflexion)[2]
  • Kalibrierung der Massenskala bei einer Konzentration von 2 pmol/μl in der Elektrospray-Massenspektromie[3]
  • Studie zur Untersuchung der Online-Ablagerung von einzelnen Tröpfchen bei der MALDI-Massenspektrometrie[4]
  • Studie zur Untersuchung der Proteinadsorption in Polyacrylamid-beschichteten Kapillaren aus synthetischem Quarzglas[5]

Biochem/physiol Actions

Myoglobin ist ein mobiler Sauerstoffträger, der in roten Muskelzellen sowie Herzzellen entsteht. Dies geschieht als Reaktion auf erhöhten Sauerstoffbedarf bei körperlicher Anstrengung; der Sauerstoff wird vom Sarkolemm zu den Mitochondrien der Herzmuskelzellen und der roten Muskelzellen von Wirbeltieren transportiert.[6]

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Dieser Artikel
M0630C7752C2506
Myoglobin aus Pferdeherz ≥90% (SDS-PAGE), essentially salt-free, lyophilized powder

Sigma-Aldrich

M1882

Myoglobin aus Pferdeherz

Myoglobin aus Pferdeskelettmuskel 95-100%, essentially salt-free, lyophilized powder

Sigma-Aldrich

M0630

Myoglobin aus Pferdeskelettmuskel

Cytochrom c aus Pferdeherz ≥95% based on Mol. Wt. 12,384 basis

Sigma-Aldrich

C7752

Cytochrom c aus Pferdeherz

Cytochrom c aus Pferdeherz ≥95% (SDS-PAGE)

Sigma-Aldrich

C2506

Cytochrom c aus Pferdeherz

technique(s)

MALDI-MS: suitable

technique(s)

mass spectrometry (MS): suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell based assay: suitable

biological source

equine heart

biological source

equine skeletal muscle

biological source

horse heart

biological source

horse heart

assay

≥90% (SDS-PAGE)

assay

95-100%

assay

≥95% (SDS-PAGE), ≥95% based on Mol. Wt. 12,384 basis

assay

≥95% (SDS-PAGE)

form

essentially salt-free, lyophilized powder

form

essentially salt-free, lyophilized powder

form

powder

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

UniProt accession no.

P68082

UniProt accession no.

P68082

UniProt accession no.

P00004

UniProt accession no.

P00004

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Lagerklasse

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Questions

1–9 of 9 Questions  

  1. Do you have the sequence for Product M1882, Myoglobin from equine heart?

    1 answer

    1. This product has not been specifically sequenced. However, according to the Uniprot accession number P68082, equine myoglobin has the following sequence:

      MGLSDGEWQQVLNVWGKVEADIAGHGQEVLIRLFTGHPETLEKFDKFKHLKTEAEMKASE DLKKHGTVVLTALGGILKKKGHHEAELKPLAQSHATKHKIPIKYLEFISDAIIHVLHSKHPGDFGADAQGAMTKALELFRNDIAAKYKELGFQG

      Please see the link below to review additional information available at UniProt:

      https://www.uniprot.org/uniprotkb/P68082/entry

      Helpful?

  2. I would like to consult you about the valence state of the ion in MB. Is it 2+ or 3+?

    1 answer

    1. This product is native myoglobin isolated from equine heart and is probably a mixture of oxidized myoglobin (metmyoglobin, Fe3+) and reduced myoglobin (Fe2+). The percentages of oxidized or reduced myoglobin in the product are not determined.

      Helpful?

  3. Was this product recombinantly expressed or was it isolated directly from the heart of the horse ?

    1 answer

    1. This product is native myoglobin isolated from equine heart.

      Helpful?

  4. Is equine myoglobin (M1882) detectable and quantifiable by common laboratory tests (ECL) for the detection of human myoglobin?

    1 answer

    1. This item has not been tested in-house for detectability and quantifiability by common laboratory tests. The specifications for this item do not include testing by these methods.

      Helpful?

  5. Is Product M1882, Myoglobin from equine heart, metmyoglobin?

    1 answer

    1. The M1882 is a mixture, but we have not analyzed the percentages of the oxidized myoglobin or the reduced myoglobin in the product.

      Helpful?

  6. Is Product M1882, Myoglobin from equine heart, oxidized?

    1 answer

    1. Yes, it is oxidized.

      Helpful?

  7. Which has more affinity for oxygen, hemoglobin or myoglobin?

    1 answer

    1. The affinity of myoglobin for oxygen is higher than that of hemoglobin.

      Helpful?

  8. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  9. What is the molecular weight of Product M1882, Myoglobin from equine heart?

    1 answer

    1. Based on the amino acid sequence (16,950) plus the heme group (616), the molecular weight is approximately 17,600 g/mol.

      Helpful?

1 Review This action will navigate to reviews.
1 of 1 reviewers received a sample product or took part in a promotion
1 out of 1 (100%) reviewers recommend this product

Reviews

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  1. New York
    • Review 1
    • Votes 0
    4 out of 5 stars.

    Is the myoglobin from equine heart dispersive in water?
    Or what are the solvent where it is dispersive.

    Helpful?

    1. Response from Merck KGaA:

      Thank you for your review. We encourage customers who experience problems with our products to call or email us for additional technical support. Please visit https://www.sigmaaldrich.com/US/en/support/customer-support to submit a Product Technical Inquiry.