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L8258

Lectin from Wisteria floribunda

lyophilized powder

Synonym(e):

Wisteria floribunda agglutinin, WFA

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PackungsgrößeSKUVerfügbarkeitPreis
1 mg
Warenkorb auf Verfügbarkeit prüfen
€ 84,20
5 mg
Warenkorb auf Verfügbarkeit prüfen
€ 278,00
€ 236,30

Über diesen Artikel

UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
MFCD00166187
Form:
lyophilized powder

€ 84,20

Warenkorb auf Verfügbarkeit prüfen
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Unterstützung erhalten

form

lyophilized powder

Quality Level

200

potency

<16 μg/mL agglutination activity

composition

Protein, ~95% E1%/280

storage temp.

−20°C

General description

Lectin is a carbohydrate-binding protein found in plant roots, rhizomes, leaves, seeds, and stems.[1][2] Lectin is located in the cytoplasm as well as in the nucleus.[2]

Application

Lectin from Wisteria floribunda has been used:
  • to study the perineuronal nets and parvalbumin nets in mouse brain by immunofluorescence[3]
  • to evaluate the amount of N-acetylgalactosamine (GalNAc) transferred to Gaussia luciferase (GLuc) substrates by microplate assay[4]
  • to study its binding effects on perineuronal nets[5]

Biochem/physiol Actions

Lectin binds with high affinity to the glycans of polysaccharides, glycolipids, and glycoproteins.[1] It is involved in plant defense.[2]
WFA is not blood group specific, but has an affinity for N-acetyl-D-galactosamine.

Packaging

Package size based on protein content

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hr incubation at 25 °C.

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Dieser Artikel
L1516L289561767
Lectin from Wisteria floribunda lyophilized powder

Sigma-Aldrich

L8258

Lectin from Wisteria floribunda

Lectin from Wisteria floribunda buffered aqueous solution, biotin conjugate

Sigma-Aldrich

L1516

Lectin from Wisteria floribunda

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) Isolectin B4 (BSI-B4), FITC conjugate, lyophilized powder

Sigma-Aldrich

L2895

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)

Lectin from Triticum vulgaris lyophilized, &#8805;55% (GE), powder

Sigma-Aldrich

61767

Lectin from Triticum vulgaris

form

lyophilized powder

form

buffered aqueous solution

form

lyophilized powder

form

powder

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

potency

<16 μg/mL agglutination activity

potency

-

potency

<50 μg per mL agglutination activity (using human blood group B erythrocytes)

potency

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

composition

Protein, ~95% E1%/280

composition

-

composition

Protein, ≥70% Lowry (, balance sodium citrate)

composition

-

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Lagerklasse

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
0000542320
0000542320
0000494890
0000494890
0000260480

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Artikel

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Nausicaä Lannoo et al.
Frontiers in plant science, 5, 397-397 (2014-08-29)
Plants are under constant attack from pathogens and herbivorous insects. To protect and defend themselves, plants evolved a multi-layered surveillance system, known as the innate immune system. Plants sense their encounters upon perception of conserved microbial structures and damage-associated patterns
Lectins, lectin genes, and their role in plant defense.
M J Chrispeels et al.
The Plant cell, 3(1), 1-9 (1991-01-01)
Dorothy Fiete et al.
The Journal of biological chemistry, 287(34), 29194-29203 (2012-06-23)
Two closely related β1,4-N-acetylgalactosaminyltransferases, β4GalNAc-T3 and β4GalNAc-T4, are thought to account for the protein-specific addition of β1,4-linked GalNAc to Asn-linked oligosaccharides on a number of glycoproteins including the glycoprotein hormone luteinizing hormone and carbonic anhydrase-6 (CA6). We have utilized soluble
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Global Trade Item Number

SKUGTIN
L8258-1MG04061826683682
L8258-5MG04061833272572

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